Teale J M
J Immunol. 1985 Aug;135(2):954-8.
The appearance of functional, antigen-specific B cells was studied in an in vitro fetal organ culture system in the absence of environmental influences associated with circulation and cell migration. In this way the B cell diversification process could be analyzed when genetic influences were dominating. By using this system in combination with the splenic focus assay, the frequency of developing B cells responsive to a number of hapten probes was measured. The results indicate that B cell diversification in vitro, in the apparent absence of many environmental influences, results in the appearance of antigen-responsive B cells in a predictable, temporal order. The results suggest that the acquisition of the expressed repertoire to a large extent is genetically controlled.
在不存在与循环和细胞迁移相关的环境影响的体外胎儿器官培养系统中，研究了功能性、抗原特异性B细胞的出现情况。通过这种方式，当遗传影响占主导时，就可以分析B细胞多样化过程。将该系统与脾集落测定法结合使用，测量了对多种半抗原探针有反应的发育中B细胞的频率。结果表明，在明显缺乏许多环境影响的情况下，体外B细胞多样化会以可预测的时间顺序导致抗原反应性B细胞的出现。结果表明，所表达的库的获得在很大程度上受基因控制。
