Krevolin M D, Calendar R
J Mol Biol. 1985 Apr 20;182(4):509-17. doi: 10.1016/0022-2836(85)90237-2.
A soluble enzyme system has been prepared from a phage P4-infected Escherichia coli strain that supports the replication of exogenous, supercoiled P4 DNA. This DNA synthesis in vitro depends upon the four deoxyribonucleotides and ATP, but is enhanced about four- to fivefold by the presence of other ribonucleotides. E. coli DNA polymerase III holoenzyme, the E. coli single-strand DNA binding protein, and the partially purified P4 alpha gene product are required for replication in vitro. Rifamycin does not inhibit P4 replication in vitro. Since the P4 alpha gene codes for a rifamycin-resistant RNA polymerase (Barrett et al., 1983), and since P4 DNA replication is independent of the host primase (Bowden et al., 1975), we believe the alpha gene product is functioning as a P4-specific DNA primase.
已从噬菌体P4感染的大肠杆菌菌株中制备出一种可溶性酶系统，该系统支持外源超螺旋P4 DNA的复制。这种体外DNA合成依赖于四种脱氧核糖核苷酸和ATP，但其他核糖核苷酸的存在可使其增强约四至五倍。体外复制需要大肠杆菌DNA聚合酶III全酶、大肠杆菌单链DNA结合蛋白和部分纯化的P4α基因产物。利福平不抑制体外P4复制。由于P4α基因编码一种利福平抗性RNA聚合酶（巴雷特等人，1983年），且由于P4 DNA复制不依赖于宿主引发酶（鲍登等人，1975年），我们认为α基因产物作为一种P4特异性DNA引发酶发挥作用。
