Ghisotti D, Finkel S, Halling C, Dehò G, Sironi G, Calendar R
Dipartimento di Genetica e di Biologia dei Microrganismi, Università degli Studi di Milano, Italy.
J Virol. 1990 Jan;64(1):24-36. doi: 10.1128/JVI.64.1.24-36.1990.
We sequenced the leftmost 2,640 base pairs of bacteriophage P4 DNA, thus completing the sequence of the 11,627-base-pair P4 genome. The newly sequenced region encodes three nonessential genes, which are called gop, beta, and cII (in order, from left to right). The gop gene product kills Escherichia coli when the beta protein is absent; the gop and beta genes are transcribed rightward from the same promoter. The cII gene is transcribed leftward to a rho-independent terminator. Mutation of this terminator creates a temperature-sensitive phenotype, presumably owing to a defect in expression of the beta gene.
我们对噬菌体P4 DNA最左边的2640个碱基对进行了测序，从而完成了11627个碱基对的P4基因组序列测定。新测序的区域编码三个非必需基因，从左到右依次称为gop、β和cII。当β蛋白不存在时，gop基因产物会杀死大肠杆菌；gop和β基因从同一个启动子向右转录。cII基因向左转录至一个不依赖ρ因子的终止子。该终止子发生突变会产生温度敏感型表型，可能是由于β基因表达缺陷所致。
