Yan Xu, Zou Ruiqi, Lin Qiqi, Ma Yuan, Li Aixin, Sun Xun, Lu Geyu, Li Hongxia
State Key Laboratory of Integrated Optoelectronics, Key Laboratory of Advanced Gas Sensors, College of Electronic Science and Engineering, Jilin University, Changchun 130012, PR China.
Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun 130062, PR China.
Food Chem. 2024 Sep 15;452:139569. doi: 10.1016/j.foodchem.2024.139569. Epub 2024 May 6.
Given the potential dangers of thiram to food safety, constructing a facile sensor is significantly critical. Herein, we presented a colorimetric sensor based on glutathione‑iron hybrid (GSH-Fe) nanozyme for specific and stable detection of thiram. The GSH-Fe nanozyme exhibits good peroxidase-mimicking activity with comparable Michaelis constant (K = 0.551 mM) to the natural enzyme. Thiram pesticides can specifically limit the catalytic activity of GSH-Fe nanozyme via surface passivation, causing the change of colorimetric signal. It is worth mentioning that the platform was used to prepare a portable hydrogel kit for rapid qualitative monitoring of thiram. Coupling with an image-processing algorithm, the colorimetric image of the hydrogel reactor is converted into the data information for accurate quantification of thiram with a detection limit of 0.3 μg mL. The sensing system has good selectivity and high stability, with recovery rates in fruit juice samples ranging from 92.4% to 106.9%.
鉴于福美双对食品安全存在潜在危害，构建一种简便的传感器至关重要。在此，我们展示了一种基于谷胱甘肽-铁杂化体（GSH-Fe）纳米酶的比色传感器，用于特异性和稳定地检测福美双。GSH-Fe纳米酶表现出良好的过氧化物酶模拟活性，其米氏常数（K = 0.551 mM）与天然酶相当。福美双农药可通过表面钝化特异性地限制GSH-Fe纳米酶的催化活性，导致比色信号发生变化。值得一提的是，该平台用于制备便携式水凝胶试剂盒，用于福美双的快速定性监测。结合图像处理算法，水凝胶反应器的比色图像被转换为数据信息，用于福美双的准确定量，检测限为0.3 μg mL。该传感系统具有良好的选择性和高稳定性，果汁样品中的回收率在92.4%至106.9%之间。
