Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Dokl Biochem Biophys. 2024 Aug;517(1):127-133. doi: 10.1134/S1607672924700820. Epub 2024 May 14.
Transvection is a phenomenon of interallelic communication in which enhancers can activate a specific promoter located on a homologous chromosome. Insulators play a significant role in ensuring functional interactions between enhancers and promoters. In the presented work, we created a model where two or three copies of the insulator are located next to enhancers and promoters localized on homologous chromosomes. Using the Su(Hw) insulator as a model, we showed that the functional interaction between a pair of insulators promotes enhancer-promoter trans-interactions. The interaction between the three insulators, on the contrary, can lead to the formation of chromatin loops that sterically hinder the full enhancer-promoter interaction. The results of the work suggest the participation of insulators in the regulation of homologous chromosome pairing and in communication between distant genomic loci.
转导是等位基因间通讯的一种现象，其中增强子可以激活位于同源染色体上的特定启动子。绝缘子在确保增强子和启动子之间的功能相互作用方面起着重要作用。在本工作中，我们创建了一个模型，其中两个或三个绝缘子拷贝位于位于同源染色体上的增强子和启动子旁边。使用 Su(Hw)绝缘子作为模型，我们表明一对绝缘子之间的功能相互作用促进了增强子-启动子的转相互作用。相反，三个绝缘子之间的相互作用可能导致染色质环的形成，从而阻碍增强子-启动子的完全相互作用。该工作的结果表明绝缘子参与了同源染色体配对的调控以及远距离基因组位点之间的通讯。
