University of Al-Qadisiyah, College of Medicine, Department of Medical Microbiology - Diwaniya, Iraq.
Rev Assoc Med Bras (1992). 2024 May 13;70(4):e20230845. doi: 10.1590/1806-9282.20230845. eCollection 2024.
COVID-19 infection poses significant risks, including life-threatening consequences and fungus synchronization, making it a significant concern. This study seeks to assess the effect of concurrent infection of COVID-19 with Thrush Candida albicans on the patient's health state by measuring the proportion of immune cells and certain interleukins such as IL-8, -10, -17, and -33.
The study involved 70 patients (30 patients with COVID-19, 17 patients with thrush candidiasis, and 23 patients with Thrush Candida albicans) and 50 healthy individuals as a control group. COVID-19 was identified using RT-PCR, while C. albicans were identified through culture media, biochemical testing, and oral swabs. Ruby equipment and ELISA kits were used for blood counts and interleukin detection.
COVID-19, thrush candidiasis, and Thrush Candida albicans infections occur in a wide range of age groups (4-80 years), with no significant differences between sexes (p>0.05). Immunologically, our study found that Thrush Candida albicans patients had the highest rate of neutrophils (89.6%) and basophils (2.01%), while corona patients had the highest percentage of lymphocytes (70.12%) and eosinophils (7.11%), and patients with thrush candidiasis had the highest percentage of monocytes. Thrush Candida albicans patients showed increased IL-8 (56.7 pg/mL) and IL-17 (101.1 pg/mL) concentrations, with the greatest concentration of IL-33 (200.5 pg/mL) in COVID-19, and a decrease in the level of IL-10 in patient groups compared with controls.
Patient groups showed increased neutrophils, lymphocytes, monocytes, and IL-8 levels, with a significant linear association between proinflammatory interleukins and these cells.
COVID-19 感染存在重大风险，包括危及生命的后果和真菌同步感染，因此应予以高度关注。本研究旨在通过检测免疫细胞比例和某些细胞因子（如 IL-8、-10、-17 和 -33），评估 COVID-19 与白色念珠菌鹅口疮（thrush Candida albicans）合并感染对患者健康状况的影响。
本研究纳入 70 例患者（30 例 COVID-19 患者、17 例鹅口疮念珠菌病患者和 23 例白色念珠菌鹅口疮患者）和 50 例健康对照者。COVID-19 通过 RT-PCR 检测，白色念珠菌通过培养、生化检测和口腔拭子检测。使用 Ruby 设备和 ELISA 试剂盒进行白细胞计数和细胞因子检测。
COVID-19、鹅口疮念珠菌病和白色念珠菌鹅口疮感染在 4-80 岁的广泛年龄组中均有发生，不同性别之间无显著差异（p>0.05）。免疫方面，本研究发现白色念珠菌鹅口疮患者中性粒细胞（89.6%）和嗜碱性粒细胞（2.01%）比例最高，而新冠患者淋巴细胞（70.12%）和嗜酸性粒细胞（7.11%）比例最高，鹅口疮念珠菌病患者单核细胞比例最高。白色念珠菌鹅口疮患者的 IL-8（56.7 pg/mL）和 IL-17（101.1 pg/mL）浓度升高，新冠患者的 IL-33 浓度最高（200.5 pg/mL），与对照组相比，患者组的 IL-10 水平降低。
患者组的中性粒细胞、淋巴细胞、单核细胞和 IL-8 水平升高，促炎细胞因子与这些细胞呈显著线性相关。
