Inamizu T, Chang M P, Makinodan T
Immunology. 1985 Jul;55(3):447-55.
The decrease in T-cell proliferation with age is due, in part, to the decline in the production of IL-2. Since IL-1 is needed to trigger IL-2 production, we determined the IL-1 producing capacity of peritoneal macrophages of young (2-4 months) and old (24-26 months) BALB/c and C57BL/6 mice. Mice were stimulated with LPS, and their peritoneal macrophages were obtained 3 days later, purified, and assessed for IL-1 production by coculturing them with splenic T cells at a ratio of 1:5 in the presence of LPS. Supernatants were obtained 4 days later when the PGE2 and IL-2 activities were minimal and IL-1 activity maximal. IL-1 activity was assessed for their ability to augment the proliferative activity of indicator thymocytes in their response to PHA stimulation. The results revealed that (i) IL-1 production by cells of old BALB/c and C57BL/6 mice is reduced to about 40% and 30% that of young mice, respectively; (ii) indomethacin enhances IL-1 production by cells of both young and old mice to the same extent; and (iii) reduction in the IL-1 producing capacity by cells of old mice results from altered activities of both the IL-1 producing peritoneal macrophages and the augmenting T cells.
随着年龄增长，T细胞增殖能力下降，部分原因是白细胞介素-2（IL-2）产生量的减少。由于需要白细胞介素-1（IL-1）来触发IL-2的产生，我们测定了年轻（2 - 4个月）和年老（24 - 26个月）的BALB/c和C57BL/6小鼠腹膜巨噬细胞产生IL-1的能力。用脂多糖（LPS）刺激小鼠，3天后获取它们的腹膜巨噬细胞，进行纯化，并通过在LPS存在的情况下以1:5的比例将其与脾T细胞共培养来评估IL-1的产生。4天后，当前列腺素E2（PGE2）和IL-2活性最低且IL-1活性最高时获取上清液。通过评估IL-1增强指示性胸腺细胞对PHA刺激反应的增殖活性的能力来测定IL-1活性。结果显示：（i）年老的BALB/c和C57BL/6小鼠细胞产生的IL-1分别降至年轻小鼠的约40%和30%；（ii）吲哚美辛在相同程度上增强年轻和年老小鼠细胞产生IL-1的能力；（iii）年老小鼠细胞产生IL-1能力的降低是由产生IL-1的腹膜巨噬细胞和增强作用的T细胞的活性改变所致。
