Zimecki M, Wieczorek Z, Kapp J A
Department of Experimental Therapy, Polish Academy of Sciences, Czerska, Wrocław.
Arch Immunol Ther Exp (Warsz). 1994;42(3):163-9.
Coculture of paraformaldehyde-fixed macrophages with syngeneic thymocytes resulted in synthesis of a lymphokine which triggered IL-1 production in untreated, peritoneal macrophages. The release of that lymphokine was MHC restricted, however, the stimulation of IL-1 production was not MHC-controlled event. Preliminary studies showed that the lymphokine had molecular weight (MW) of around 10-30 kDa, was heat stable (56 degrees C), resistant to reduction to DTT and exhibited a weak pyrogenic activity. Fusion of activated thymocytes with the thymoma BW5147 resulted in obtaining several hybridoma clones producing the lymphokine. Preparation of culture supernatants by means of Amicon membranes and Sephadex G-100 filtration allowed to determine the MW of the lymphokine as 25 kDa. Lastly, the lymphokine was identified as IL-6 by means of anti-IL-6 antibodies which blocked its function and by the use of IL-6-sensitive cell line 7TD1.
将多聚甲醛固定的巨噬细胞与同基因胸腺细胞共培养，可导致一种淋巴因子的合成，该淋巴因子能触发未处理的腹腔巨噬细胞产生白细胞介素-1（IL-1）。然而，该淋巴因子的释放受主要组织相容性复合体（MHC）限制，而IL-1产生的刺激并非MHC控制的事件。初步研究表明，该淋巴因子的分子量（MW）约为10 - 30 kDa，热稳定（56℃），对二硫苏糖醇（DTT）还原有抗性，并表现出较弱的致热活性。活化的胸腺细胞与胸腺瘤BW5147融合，获得了几个产生该淋巴因子的杂交瘤克隆。通过Amicon膜和Sephadex G - 100过滤制备培养上清液，确定该淋巴因子的MW为25 kDa。最后，通过阻断其功能的抗IL - 6抗体以及使用对IL - 6敏感的细胞系7TD1，将该淋巴因子鉴定为IL - 6。
