Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Khon Kaen University, Khon Kaen, Thailand.
Sci Rep. 2024 May 15;14(1):11145. doi: 10.1038/s41598-024-61843-4.
The global distribution of tropical fire ants (Solenopsis geminata) raises concerns about anaphylaxis and serious medical issues in numerous countries. This investigation focused on the cross-reactivity of allergen-specific IgE antibodies between S. geminata and Myrmecia pilosula (Jack Jumper ant) venom proteins due to the potential emergence of cross-reactive allergies in the future. Antibody epitope analysis unveiled one predominant conformational epitope on Sol g 1.1 (PI score of 0.989), followed by Sol g 2.2, Sol g 4.1, and Sol g 3.1. Additionally, Pilosulin 1 showed high allergenic potential (PI score of 0.94), with Pilosulin 5a (PI score of 0.797) leading in B-cell epitopes. The sequence analysis indicated that Sol g 2.2 and Sol g 4.1 pose a high risk of cross-reactivity with Pilosulins 4.1a and 5a. Furthermore, the cross-reactivity of recombinant Sol g proteins with M. pilosula-specific IgE antibodies from 41 patients revealed high cross-reactivity for r-Sol g 3.1 (58.53%) and r-Sol g 4.1 (43.90%), followed by r-Sol g 2.2 (26.82%), and r-Sol g 1.1 (9.75%). Therefore, this study demonstrates cross-reactivity (85.36%) between S. geminata and M. pilosula, highlighting the allergenic risk. Understanding these reactions is vital for the prevention of severe allergic reactions, especially in individuals with pre-existing Jumper Jack ant allergy, informing future management strategies.
热带火蚁（Solenopsis geminata）在全球范围内的分布引起了人们对众多国家过敏反应和严重医疗问题的关注。本研究主要关注由于未来可能出现交叉过敏反应，S. geminata 和 Myrmecia pilosula（Jack Jumper 蚁）毒液蛋白之间过敏原特异性 IgE 抗体的交叉反应性。抗体表位分析揭示了 Sol g 1.1 上存在一个主要的构象表位（PI 评分为 0.989），其次是 Sol g 2.2、Sol g 4.1 和 Sol g 3.1。此外，Pilosulin 1 表现出高的过敏原性（PI 评分为 0.94），Pilosulin 5a （PI 评分为 0.797）具有更多的 B 细胞表位。序列分析表明，Sol g 2.2 和 Sol g 4.1 与 Pilosulins 4.1a 和 5a 具有很高的交叉反应性风险。此外，41 名患者的 M. pilosula 特异性 IgE 抗体与重组 Sol g 蛋白的交叉反应性研究表明，r-Sol g 3.1（58.53%）和 r-Sol g 4.1（43.90%）具有高交叉反应性，其次是 r-Sol g 2.2（26.82%）和 r-Sol g 1.1（9.75%）。因此，本研究表明 S. geminata 和 M. pilosula 之间存在交叉反应性（85.36%），突出了其致敏风险。了解这些反应对于预防严重过敏反应至关重要，特别是对于已经存在 Jumper Jack 蚁过敏的个体，这为未来的管理策略提供了信息。
