Behlke M A, Spinella D G, Chou H S, Sha W, Hartl D L, Loh D Y
Science. 1985 Aug 9;229(4713):566-70. doi: 10.1126/science.3875151.
Fifteen independently isolated complementary DNA clones that contain T-cell receptor (TCR) V beta genes were sequenced and found to represent 11 different V beta genes. When compared with known sequences, 14 different V beta genes could be defined from a total of 25 complementary DNA's; 11 clones therefore involved repeated usage of previously identified V beta's. Based on these data, we calculate a maximum likelihood estimate of the number of expressed germline V beta genes to be 18 with an upper 95 percent confidence bound of 30 genes. Southern blot analysis has shown that most of these genes belong to single element subfamilies which show very limited interstrain polymorphism. The TCR beta-chain diversity appears to be generated from a limited V beta gene pool primarily by extensive variability at the variable-diversity-joining (V-D-J) junctional site, with no evidence for the involvement of somatic hypermutation.
对15个独立分离的含有T细胞受体（TCR）Vβ基因的互补DNA克隆进行测序，发现它们代表11种不同的Vβ基因。与已知序列比较时，从总共25个互补DNA中可确定14种不同的Vβ基因；因此，11个克隆涉及先前鉴定的Vβ基因的重复使用。基于这些数据，我们计算出表达的种系Vβ基因数量的最大似然估计值为18，95%置信上限为30个基因。Southern印迹分析表明，这些基因大多数属于单元素亚家族，其种间多态性非常有限。TCRβ链多样性似乎主要由可变-多样-连接（V-D-J）连接位点的广泛变异性从有限的Vβ基因库产生，没有证据表明体细胞超突变参与其中。
