Fouché Mathieu, Bonnet Hélène, Bonnet Diane M V, Wenden Bénédicte
INRAE, Univ. Bordeaux, UMR Biologie du Fruit et Pathologie 1332, Villenave d'Ornon, France.
Front Plant Sci. 2023 Nov 14;14:1240642. doi: 10.3389/fpls.2023.1240642. eCollection 2023.
To avoid the negative impacts of winter unfavorable conditions for plant development, temperate trees enter a rest period called dormancy. Winter dormancy is a complex process that involves multiple signaling pathways and previous studies have suggested that transport capacity between cells and between the buds and the twig may regulate the progression throughout dormancy stages. However, the dynamics and molecular actors involved in this regulation are still poorly described in fruit trees.
Here, in order to validate the hypothesis that transport capacity regulates dormancy progression in fruit trees, we combined physiological, imaging and transcriptomic approaches to characterize molecular pathways and transport capacity during dormancy in sweet cherry (Prunus avium L.) flower buds.
Our results show that transport capacity is reduced during dormancy and could be regulated by environmental signals. Moreover, we demonstrate that dormancy release is not synchronized with the transport capacity resumption but occurs when the bud is capable of growth under the influence of warmer temperatures. We highlight key genes involved in transport capacity during dormancy.
Based on long-term observations conducted during six winter seasons, we propose hypotheses on the environmental and molecular regulation of transport capacity, in relation to dormancy and growth resumption in sweet cherry.
为避免冬季不利条件对植物发育产生负面影响，温带树木会进入一个称为休眠的静止期。冬季休眠是一个复杂的过程，涉及多个信号通路，先前的研究表明，细胞之间以及芽与嫩枝之间的运输能力可能会调节整个休眠阶段的进程。然而，在果树中，参与这种调节的动态变化和分子作用因子仍鲜为人知。
在此，为了验证运输能力调节果树休眠进程这一假设，我们结合了生理学、成像和转录组学方法，以表征甜樱桃（Prunus avium L.）花芽休眠期间的分子途径和运输能力。
我们的结果表明，休眠期间运输能力降低，并且可能受环境信号调节。此外，我们证明休眠解除与运输能力恢复不同步，而是在芽在温暖温度影响下能够生长时发生。我们突出了休眠期间参与运输能力的关键基因。
基于在六个冬季进行的长期观察，我们提出了关于甜樱桃运输能力的环境和分子调节与休眠及生长恢复相关的假设。
