Hoskins J D, Newman S S, Roy A F, Foil C S, Cox H U
Am J Vet Res. 1985 Jul;46(7):1526-8.
One iodometric, 2 chromogenic, and 3 acidometric methods were compared for the detection of beta-lactamase produced by Staphylococcus intermedius (n = 105) isolated from dogs. Of 575 tests performed, using the 6 methods evaluated, 316 (55.0%) were positive for beta-lactamase production. The iodometric method was the reference method. With the exception of a high correlation (r = 0.962) between 1 acidometric method and 1 chromogenic method, the 5 commercial methods had correlation coefficients less than 0.900 when comparisons were made among them. The 6 methods were in agreement for 69 (65.7%) of the isolates. Based on the findings of this study, an inexpensive, laboratory-prepared, paper strip iodometric method was as reliable as 5 commercial methods for beta-lactamase detection and is recommended for routine use in clinical laboratories.
比较了1种碘量法、2种显色法和3种酸碱滴定法，用于检测从犬类分离的中间葡萄球菌（n = 105）产生的β-内酰胺酶。在使用评估的6种方法进行的575次检测中，316次（55.0%）β-内酰胺酶产生检测呈阳性。碘量法为参考方法。除1种酸碱滴定法和1种显色法之间具有高度相关性（r = 0.962）外，5种商业方法之间比较时，相关系数均小于0.900。6种方法对69株（65.7%）分离株的检测结果一致。基于本研究结果，一种廉价的、实验室自制的纸条碘量法在检测β-内酰胺酶方面与5种商业方法一样可靠，推荐在临床实验室常规使用。
