Lee D T, Rosenblatt J E
Diagn Microbiol Infect Dis. 1983 Jun;1(2):173-5. doi: 10.1016/0732-8893(83)90048-2.
Four methods for detecting beta-lactamase activity in anaerobic bacteria were evaluated and compared to a nitrocefin saturated filter paper test. The methods studied were Cefinase, PADAC, Beta Lactam, and the slide iodometric technique. Fifty anaerobes were tested including species of Bacteroides, Fusobacterium, and Clostridium. The percentages of agreement with the nitrocefin test are: Cefinase 100%, PADAC 96%, slide iodometric 78%, and Beta Lactam 72%. Positive nitrocefin tests have been shown to correlate with a penicillin MIC greater than 0.78 micrograms/ml.
对检测厌氧菌中β-内酰胺酶活性的四种方法进行了评估，并与硝基头孢菌素饱和滤纸试验进行了比较。所研究的方法有头孢菌素酶试验、PADAC试验、β-内酰胺试验和玻片碘量法。对50株厌氧菌进行了检测，包括拟杆菌属、梭杆菌属和梭菌属的菌种。与硝基头孢菌素试验的符合率分别为：头孢菌素酶试验100%、PADAC试验96%、玻片碘量法78%、β-内酰胺试验72%。已证明硝基头孢菌素试验阳性与青霉素最低抑菌浓度大于0.78微克/毫升相关。
