纳米脂质体负载 Mito-Tempo 对人精子冷冻保存过程中精子参数的评估效果。
The evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation.
机构信息
Department of Reproductive Biology, Academic Center for Education, Culture, and Research (ACECR), Qom, Iran.
Infertility Treatment Center of ACECR, Bonyad Street, P.O. box: 3713746611, Qom, Iran.
出版信息
J Assist Reprod Genet. 2024 Aug;41(8):2053-2063. doi: 10.1007/s10815-024-03132-7. Epub 2024 May 16.
AIM
The aim of this study is the evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation.
METHODS
Semen samples of 50 Asthenoteratozoospermia men (random) were collected. Sperm parameters were analyzed based on World Health Organization (WHO, 2010) criteria (2021) and each sample was divided into 5 groups (E1-E5). E1 (control group): the sperm was cryopreserved without nanoliposome, and Mito-Tempo. E2: sperm cryopreservation with Mito-Tempo-loaded nanoliposome (Mito-Tempo 0.1 mM) + freezing medium. E3: sperm cryopreservation with Mito-Tempo-loaded nanoliposome (Mito-Tempo 0.2 mM) + freezing medium. E4: in this group, the cryopreservation sperm with Mito-Tempo 0.3 mM + freezing medium. E5: the cryopreservation sperm with Mito-Tempo 0.2 mM + freezing medium.
RESULTS
The result of this study indicated that sperm parameters and total antioxidant capacity (TAC) significantly increase in E3 and E4 groups, compared to E1, E2, and E5 groups respectively (P < 0.05). The percentage of abnormal morphology, DNA fragmentation index (DFI), malondialdehyde (MDA), and the levels of ROS significantly decrease in E3 and E4 groups, compared to E1, E2, and E5 groups (P < 0.05). In addition, the sperm parameters and stress oxidative factors significantly improve in E3 group compared to other groups (P < 0.05).
CONCLUSIONS
In conclusion, the combination of Mito-Tempo with nanoliposome due to its ability to cooperate with lipid layers may lead to significant performance in reducing oxidative stress damage and increasing the quality of sperm parameters.
目的
本研究旨在评估载米托蒽醌纳米脂质体对人精子冷冻保存过程中精子参数的影响。
方法
收集 50 例弱畸精子症患者（随机）的精液样本。根据世界卫生组织（WHO，2010 年）标准（2021 年）分析精子参数，每个样本分为 5 组（E1-E5）。E1（对照组）：精子未经纳米脂质体和米托蒽醌冷冻保存。E2：含米托蒽醌纳米脂质体（米托蒽醌 0.1mM）+冷冻液的精子冷冻保存。E3：含米托蒽醌纳米脂质体（米托蒽醌 0.2mM）+冷冻液的精子冷冻保存。E4：在本研究中，用米托蒽醌 0.3mM+冷冻液冷冻保存精子。E5：用米托蒽醌 0.2mM+冷冻液冷冻保存精子。
结果
研究结果表明，与 E1、E2 和 E5 组相比，E3 和 E4 组的精子参数和总抗氧化能力（TAC）显著增加（P<0.05）。与 E1、E2 和 E5 组相比，E3 和 E4 组的异常形态百分比、DNA 碎片指数（DFI）、丙二醛（MDA）和 ROS 水平显著降低（P<0.05）。此外，与其他组相比，E3 组的精子参数和应激氧化因子显著改善（P<0.05）。
结论
总之，米托蒽醌与纳米脂质体的结合可能由于其与脂质层的协同作用，导致在降低氧化应激损伤和提高精子参数质量方面具有显著的性能。
Zotero 插件