Loudi Vocational and Technical College, Loudi, 417000, China.
Cell Biochem Biophys. 2024 Jun;82(2):1503-1510. doi: 10.1007/s12013-024-01303-y. Epub 2024 May 16.
Puerarin (Pue), a flavonoid compound, possesses cytoprotective effects and LPS has been reported to induce renal inflammatory injury in bovine. However, whether Pue inhibits lipopolysaccharide (LPS)-induced inflammatory damage of bovine kidney cells remains unknown. Based on an in vitro model with Madin-Darby bovine kidney (MDBK) cell line, it has found that Pue attenuated LPS-induced damage of MDBK cells, as evidenced by cell viability and lactic dehydrogenase (LDH) release rescued by Pue (P < 0.05). Additionally, the real-time quantitative PCR (qPCR) and enzyme linked immunosorbent assay (ELISA) showed that LPS elevated the levels of pro-inflammatory factors interleukin (IL)-1β, IL-8 and tumor necrosis factor (TNF)-α, which was reversed by pretreatment of Pue (P < 0.05). Besides, Pue reduced the expression of Toll like receptor 4 (TLR4) and phosphorylated nuclear factor kappa B (p-NF-κB) of LPS-exposed MDBK cells (P < 0.05). Collectively, these results showed that Pue suppresses LPS-evoked inflammatory damage of bovine kidney cells, suggesting Pue a potential compound for intervention of bovine inflammation.
葛根素（Pue）是一种黄酮类化合物，具有细胞保护作用，已有报道称脂多糖（LPS）可诱导牛肾的炎症损伤。然而，葛根素是否抑制脂多糖（LPS）诱导的牛肾细胞炎症损伤尚不清楚。基于体外 Madin-Darby 牛肾（MDBK）细胞系模型，发现葛根素减轻了 LPS 诱导的 MDBK 细胞损伤，葛根素可挽救细胞活力和乳酸脱氢酶（LDH）释放（P<0.05）。此外，实时定量 PCR（qPCR）和酶联免疫吸附试验（ELISA）显示，LPS 上调了促炎因子白细胞介素（IL）-1β、IL-8 和肿瘤坏死因子（TNF）-α的水平，而葛根素预处理可逆转这一现象（P<0.05）。此外，葛根素降低了 LPS 暴露的 MDBK 细胞中 Toll 样受体 4（TLR4）和磷酸化核因子 kappa B（p-NF-κB）的表达（P<0.05）。综上所述，这些结果表明葛根素抑制 LPS 诱导的牛肾细胞炎症损伤，提示葛根素可能是一种干预牛炎症的潜在化合物。
