Chen Huan, Peng Tao, Shang Hanle, Shang Xianglong, Zhao Xianghui, Qu Mingren, Song Xiaozhen
Jiangxi Province Key Laboratory of Animal Nutrition, Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang, China.
Front Nutr. 2022 Mar 21;9:817557. doi: 10.3389/fnut.2022.817557. eCollection 2022.
To investigate the effect of on intramuscular fat deposition in heat-stressed beef cattle and its underlying mechanism. Thirty-two healthy Jinjiang bulls were randomly divided into four groups and dietary with 0 (Control), 200 (Pue200), 400 (Pue400), and 800 (Pue800) mg/kg in the feed concentrate. The results showed that treatment enhanced the concentration of crude fat, fatty acid (C14:1 and C17:1), and the activity of fatty acid synthase in (LT), but decreased the levels of blood leptin ( < 0.05). High-throughput sequencing of mRNA technology (RNA-Seq) was used and the analysis showed that 492 genes were down-regulated and 341 genes were up-regulated in LT, and these genes were significantly enriched to the pathways related to lipid metabolism. These results indicated that dietary supplemental with enhanced intramuscular fat deposition by regulating lipid metabolism of heat-stressed beef cattle.
为研究[物质名称]对热应激肉牛肌内脂肪沉积的影响及其潜在机制。将32头健康的晋江公牛随机分为四组，在精饲料中分别添加0（对照组）、200（Pue200组）、400（Pue400组）和800（Pue800组）mg/kg的[物质名称]。结果表明，[物质名称]处理提高了热应激（LT）肉牛的粗脂肪、脂肪酸（C14:1和C17:1）浓度以及脂肪酸合酶活性，但降低了血液瘦素水平（P<0.05）。采用mRNA高通量测序技术（RNA-Seq）分析发现，热应激肉牛中有492个基因下调，341个基因上调，这些基因显著富集于脂质代谢相关通路。这些结果表明，日粮中添加[物质名称]可通过调节热应激肉牛的脂质代谢来增强肌内脂肪沉积。
