Laohapand T, Smith J, Cattell V
Br J Exp Pathol. 1985 Aug;66(4):475-82.
Monocytes infiltrate glomeruli during mesangial deposition of ferritin, and during experimental glomerulonephritis. To determine whether this is solely a local phenomenon, leucocyte infiltration in other organs has been studied following intravenous ferritin injection. Lewis rats received an i.v. injection of 150 mg ferritin/100 g body weight. At 24 h there was a peripheral blood leucocytosis (ferritin-treated rats 26.32 +/- 13.7, control rats 8.54 +/- 2.41 X 10(6) cells/ml) due to increase in polymorphs and monocytes. Bone marrow cell counts fell (ferritin-treated rats 49 +/- 7, control 80 +/- 11 X 10(6)/100 g body weight). Cell counts on cell suspensions of perfused, enzyme-digested lung, liver and spleen, and lung lavage showed major significant increases in total cell counts: lung 250 +/- 36 (89 +/- 16), lung lavage 2.6 +/- 0.8 (1.4 +/- 0.5), liver 140 +/- 37 (60 +/- 11), spleen 306 +/- 38 (200 +/- 27) X 10(6)/100 g body weight (control values in parentheses). Cytospin preparations of these suspensions, stained for non-specific esterase showed that the increase in cell numbers was due to increases in non-specific esterase-positive cells (monocytes) and polymorphs. These results demonstrate a generalized leucocyte mobilization, sequestration, and tissue infiltration after i.v. ferritin. The renal glomerulus therefore is not the only site of leucocyte accumulation. These findings may have relevance for studies on inflammation mediated by leucocytes in models of experimental immune complex glomerulonephritis.
在铁蛋白系膜沉积期间以及实验性肾小球肾炎期间，单核细胞浸润肾小球。为了确定这是否仅仅是一种局部现象，在静脉注射铁蛋白后，对其他器官中的白细胞浸润情况进行了研究。Lewis大鼠接受了静脉注射150mg铁蛋白/100g体重。24小时时出现外周血白细胞增多（铁蛋白处理组大鼠为26.32±13.7，对照组大鼠为8.54±2.41×10⁶个细胞/ml），这是由于多形核白细胞和单核细胞增多所致。骨髓细胞计数下降（铁蛋白处理组大鼠为49±7，对照组为80±11×10⁶/100g体重）。对灌注、酶消化后的肺、肝和脾的细胞悬液以及肺灌洗的细胞计数显示，总细胞计数有显著大幅增加：肺为250±36（89±16），肺灌洗为2.6±0.8（1.4±0.5），肝为140±37（60±11），脾为306±38（200±27）×10⁶/100g体重（括号内为对照值）。对这些悬液进行细胞涂片制备，用非特异性酯酶染色显示，细胞数量增加是由于非特异性酯酶阳性细胞（单核细胞）和多形核白细胞增多所致。这些结果表明静脉注射铁蛋白后出现了全身性白细胞动员、滞留和组织浸润。因此，肾小球不是白细胞积聚的唯一部位。这些发现可能与实验性免疫复合物肾小球肾炎模型中白细胞介导的炎症研究相关。
