Cattell V, Jamieson S W
Lab Invest. 1978 Dec;39(6):584-90.
The origin of glomerular crescents and diffuse hypercellularity was investigated in rabbits receiving a single intravenous injection of nephrotoxic serum. Experiments to demonstrate local glomerular proliferation included mitotic counting at intervals after nephrotoxic serum and autoradiography of kidneys 1 hour after pulse-tritiated thymidine labeling. There was an increase in local endocapillary proliferation between days 4 and 6 when severe diffuse hypercellularity developed. From days 8 to 13 during crescent formation there was considerable local epithelial proliferation. To assess the role of infiltrating bone marrow cells a new technique of renal transplantation in rabbits was developed. Kidneys from unlabeled donors were transplanted on either day 4 or day 9 into recipients prelabeled with tritiated thymidine, and labeled cells were sought in glomeruli of transplants 1 to 4 days later. Large numbers of labeled cells appeared on days 5 to 6 in areas of endocapillary hypercellularity in glomeruli of animals given transplants on day 4 indicating a significant influx of bone marrow cells. In contrast, in kidneys transplanted on day 9, few labeled cells were present in crescents on days 10 to 11. Autoradiographic labeling of mononuclear phagocytes in carrageenan granulomas and interstitial renal infiltrates in recipients were used as positive controls of bone marrow cell labeling. We conclude that in the early phase of diffuse hypercellularity there is considerable blood monocyte infiltration, whereas crescents which develop later are principally the result of glomerular epithelial cell proliferation.
对接受单次静脉注射肾毒性血清的家兔肾小球新月体和弥漫性细胞增多的起源进行了研究。证明局部肾小球增殖的实验包括在注射肾毒性血清后定期进行有丝分裂计数，以及在脉冲标记氚化胸腺嘧啶核苷1小时后对肾脏进行放射自显影。在第4天至第6天，当出现严重的弥漫性细胞增多时，局部毛细血管内增殖增加。在第8天至第13天新月体形成期间，有相当数量的局部上皮细胞增殖。为了评估浸润性骨髓细胞的作用，开发了一种家兔肾移植新技术。将来自未标记供体的肾脏在第4天或第9天移植到预先用氚化胸腺嘧啶核苷标记的受体中，并在1至4天后在移植肾的肾小球中寻找标记细胞。在第4天接受移植的动物的肾小球毛细血管内细胞增多区域，在第5天至第6天出现了大量标记细胞，这表明骨髓细胞有显著流入。相比之下，在第9天移植的肾脏中，在第10天至第11天新月体中几乎没有标记细胞。将角叉菜胶肉芽肿中单核吞噬细胞的放射自显影标记以及受体肾间质浸润作为骨髓细胞标记的阳性对照。我们得出结论，在弥漫性细胞增多的早期阶段，有相当数量的血液单核细胞浸润，而后期形成的新月体主要是肾小球上皮细胞增殖的结果。
