Valone F H, Payan D G
Cancer Res. 1985 Sep;45(9):4128-31.
The capacity of retinoic acid to modulate human T-lymphocyte and B-lymphocyte activation by mitogens was examined. T-lymphocyte proliferation stimulated by 12-O-tetradecanoylphorbol-13-acetate (TPA) or phytohemagglutinin was enhanced by 5 nM to 5 microM retinoic acid in a dose-dependent manner with a 65 +/- 35% (SD) increase (n = 6, P less than 0.01) in TPA-stimulated proliferation induced by 5 microM retinoic acid. Retinoic acid enhanced T-lymphocyte proliferation over a wide range of background proliferation induced by different TPA concentrations. Retinoic acid alone did not stimulate T-lymphocyte proliferation. In contrast retinoic acid inhibited B-lymphocyte proliferation stimulated by TPA or phytohemagglutinin with 26.7 +/- 23.4% inhibition of TPA-stimulated proliferation induced by 5 microM retinoic acid (P less than 0.02). Retinoic acid had intermediate effects on the proliferation of different mixtures of T- and B-lymphocytes stimulated by TPA or phytohemagglutinin. The recognition that retinoic acid has opposing effects on human T- and B-lymphocyte activation by mitogens may account for the conflicting reports of the effects of retinoids on the immune response of unpurified human lymphocyte preparations.
研究了视黄酸调节丝裂原激活人T淋巴细胞和B淋巴细胞的能力。12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）或植物血凝素刺激的T淋巴细胞增殖，在5 nM至5 μM视黄酸作用下呈剂量依赖性增强，5 μM视黄酸诱导的TPA刺激增殖增加65±35%（标准差）（n = 6，P < 0.01）。视黄酸在由不同TPA浓度诱导的广泛背景增殖范围内增强T淋巴细胞增殖。视黄酸单独不刺激T淋巴细胞增殖。相反，视黄酸抑制TPA或植物血凝素刺激的B淋巴细胞增殖，5 μM视黄酸诱导的TPA刺激增殖抑制26.7±23.4%（P < 0.02）。视黄酸对TPA或植物血凝素刺激的不同T淋巴细胞和B淋巴细胞混合物的增殖有中等程度影响。视黄酸对丝裂原激活的人T淋巴细胞和B淋巴细胞具有相反作用这一认识，可能解释了关于类视黄醇对未纯化人淋巴细胞制剂免疫反应影响的相互矛盾的报道。
