Bi Xiaoyang, Shi Zhifei, Li Dongqin, Zhan Wenhui, Wang Zhixiong, Li Huashou, Zhao Benliang, Chen Guikui
Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
Institute of Quality Standard and Monitoring Technology for Agro-products of Guangdong Academy of Agricultural Sciences, Guangzhou 501640, China.
Ecotoxicol Environ Saf. 2025 Jan 1;289:117688. doi: 10.1016/j.ecoenv.2025.117688. Epub 2025 Jan 7.
This study aimed to investigate the tissue-specific accumulation patterns of arsenic (As) and the potential toxicological effects of As on the oviposition of a globally distributed aquatic invertebrate, the apple snail (Pomacea canaliculata). An eight-compartment physiologically based pharmacokinetic (PBPK) model was utilized to simulate the distribution and depuration kinetics of arsenite and arsenate in the snails. Modeling and biotransformation suggested that intestine-stomach was the main uptake site for As and plays an important role in maintaining the balance of As species. Liver and kidney were the accumulation and biotransformation sites, while shell was the main storage site. Moreover, compared with arsenite, arsenate was more prone to accumulate in the organs (intestine-stomach, head-foot, kidney, gonad, shell, heart, and hemolymph). Excretion through excrement is likely the primary mechanism contributing to the low accumulation of As and the subsequent rapid decline in As concentrations observed in various organs during the depuration period. Furthermore, our findings revealed a stimulating effect of As on the oviposition of P. canaliculata. Specifically, a concentration of 500 μg L of arsenite in water significantly increased the oviposition rate by 36.88 %. These findings suggest a behavioral response and changes in reproductive patterns under As exposure. The increased egg production observed in response to As exposure provides insights into the adaptive mechanisms of this species in contaminated habitats.
本研究旨在调查砷（As）在组织中的特异性积累模式以及As对全球分布的水生无脊椎动物福寿螺（Pomacea canaliculata）产卵的潜在毒理学影响。利用一个八室生理药代动力学（PBPK）模型来模拟亚砷酸盐和砷酸盐在福寿螺中的分布和净化动力学。建模和生物转化表明，肠胃是As的主要摄取部位，在维持As物种平衡中起重要作用。肝脏和肾脏是积累和生物转化部位，而外壳是主要储存部位。此外，与亚砷酸盐相比，砷酸盐更倾向于在器官（肠胃、头足、肾脏、性腺、外壳、心脏和血淋巴）中积累。通过粪便排泄可能是导致As积累量低以及在净化期各器官中As浓度随后迅速下降的主要机制。此外，我们的研究结果揭示了As对福寿螺产卵有刺激作用。具体而言，水中500μg/L的亚砷酸盐浓度显著提高了产卵率36.88%。这些发现表明在As暴露下存在行为反应和繁殖模式变化。观察到的As暴露导致的产卵增加为该物种在受污染栖息地的适应机制提供了见解。
