Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
Institute of Quality Standard and Monitoring Technology for Agro-products of Guangdong Academy of Agricultural Sciences, Guangzhou 501640, China.
J Hazard Mater. 2024 Dec 5;480:136034. doi: 10.1016/j.jhazmat.2024.136034. Epub 2024 Oct 2.
An eight-compartment physiologically based pharmacokinetic (PBPK) model was used to simulate the bioaccumulation and distribution of arsenic (As) within the apple snail (Pomacea canaliculata) following the ingestion of As-contaminated lettuce. The bioaccumulation results revealed that the shell contained the majority (67.21 %) of the total As content, with the liver and the head-foot containing approximately 11.14 % and 10.45 % of the total As content in the snail, respectively. Modeling quantified the process of intestine-stomach absorption of dietborne As and revealed its crucial role in the subsequent distribution of As within the body. The liver is the primary metabolic site, whereas the shell is the primary storage site. Exposure to dietborne As leads to pronounced physiological and biochemical alterations in apple snails. Total protein levels decreased by 24.06 %, superoxide dismutase (SOD) activity decreased by 24.43 %, malondialdehyde (MDA) content increased by 47.51 %, glutathione (GSH) content decreased by 46.99 %, and glutathione S-transferase (GST) activity decreased by 42.22 %. Furthermore, the subcellular-level results indicated that dietborne As exposure altered subcellular distribution in the liver. Additionally, dietborne As exposure significantly reduced the abundance of gut microbiota in apple snails.
采用包含 8 个隔室的生理药代动力学（PBPK）模型，模拟了苹果蜗牛（Pomacea canaliculata）在摄入含砷污染生菜后体内砷的生物累积和分布。生物累积结果表明，外壳包含了大部分（67.21%）的总砷含量，肝脏和头足分别包含了蜗牛体内总砷含量的约 11.14%和 10.45%。建模量化了肠道-胃对饮食中砷的吸收过程，并揭示了其在体内砷随后分布中的关键作用。肝脏是主要的代谢部位，而外壳是主要的储存部位。暴露于饮食中的砷会导致苹果蜗牛发生明显的生理和生化变化。总蛋白水平下降了 24.06%，超氧化物歧化酶（SOD）活性下降了 24.43%，丙二醛（MDA）含量增加了 47.51%，谷胱甘肽（GSH）含量下降了 46.99%，谷胱甘肽 S-转移酶（GST）活性下降了 42.22%。此外，亚细胞水平的结果表明，饮食中的砷暴露改变了肝脏中的亚细胞分布。此外，饮食中的砷暴露显著降低了苹果蜗牛肠道微生物群的丰度。
