Euteneuer U, Ris H, Borisy G G
Eur J Cell Biol. 1985 May;37:149-55.
Erythrocytes from three different vertebrate species (bullfrog, mudpuppy, and stinkpot) were isolated, and the polarity of the marginal-band microtubules was determined using a modification of the method described by Heidemann and McIntosh [12]. A considerable part of each marginal band was examined in serial sections to see whether any changes in the polarity pattern occurred along the length of the band. On the basis of analyses of over 45 marginal bands, the following observations were made: (i) different polarity patterns were present within a given species; (ii) the polarity pattern seen in one cross-section of the marginal band remained the same along its entire length; no evidence for a limited zone of overlap was obtained; (iii) centrioles were found in erythrocytes from all three species. The data on the polarity patterns and the presence of centrioles in erythrocytes are compatible with a model recently proposed for the organization of marginal-band microtubules in blood clams that invokes the activity of a microtubule organizing center [20]. We suggest that a modification of the process of marginal-band formation described in that model can account for our observations on the polarity of marginal-band microtubules.
分离出三种不同脊椎动物（牛蛙、泥螈和麝香龟）的红细胞，并采用对Heidemann和McIntosh [12]所描述方法的改良方法来确定边缘带微管的极性。在连续切片中检查每个边缘带的相当一部分，以观察沿带的长度方向极性模式是否发生任何变化。基于对超过45个边缘带的分析，得出以下观察结果：(i) 给定物种内存在不同的极性模式；(ii) 在边缘带的一个横截面上看到的极性模式沿其整个长度保持不变；未获得有限重叠区域的证据；(iii) 在所有三种物种的红细胞中都发现了中心粒。关于红细胞中极性模式和中心粒存在的数据与最近提出的一种模型相符，该模型用于解释血蛤中边缘带微管的组织方式，该模型涉及微管组织中心的活动 [20]。我们认为，该模型中描述的边缘带形成过程的一种修改可以解释我们对边缘带微管极性的观察结果。
