Coiro J R, Brunner A, Mitsutani C Y, Weisz V M, Fiori A M
Arch Anat Microsc Morphol Exp. 1978;67(2):133-43.
Erythrocytes from a chelonian (Geochelone carbonaria) were submitted to physical and chemical treatments. Observations of their change in shape from ellipsoidal to spherical were made by means of light microscopy. A 100% change to the spherical shape was attained with erythrocytes under high temperature treatment (42 degrees C) for 45 min., and at room temperature (29 degrees C) plus colchicine, for 60 min. Erythrocytes kept at room temperature present changes in shape while those kept at low temperature (10 degrees C) did not exhibit significant changes in their original ellipsoidal shape, but displayed an intact marginal band (MB). By direct examination the diameter of microtubules was found to be nearly 300 A in the dissociated form and 0.70 mu when bundled. In cross section the value found for microtubule diameter was up to 210 A. These measures are compatible with those found by several other authors. Counting of microtubules by ultrathin sectioning indicated approximately 70 tubules per cell, a value that is higher than that found in other species. By means of electron microscopy the marginal band can be observed intact or dissociated into several dense rings formed by parallel microtubules running nearly or accompanying the periphery of the stroma. Temperature and colchicine are responsible for the depolymerization of the microtubules. In the stroma of spherical erythrocytes a dense peripheric fold can be observed but microtubules were never found. It is suggested therefore that the role of the marginal band is essential to maintain the ellipsoidal shape of chelonian erythrocytes.
将一只陆龟（红腿陆龟）的红细胞进行物理和化学处理。通过光学显微镜观察其形状从椭圆形变为球形的变化。在高温处理（42摄氏度）45分钟以及室温（29摄氏度）加秋水仙碱处理60分钟的情况下，红细胞可100%变为球形。室温下保存的红细胞会发生形状变化，而低温（10摄氏度）保存的红细胞其原始椭圆形形状未出现明显变化，但边缘带（MB）保持完整。直接检查发现，微管解离形式下的直径约为300埃，成束时为0.70微米。在横切面上，微管直径的值可达210埃。这些测量结果与其他几位作者的发现相符。通过超薄切片对微管进行计数表明，每个细胞约有70根微管，该值高于在其他物种中发现的值。通过电子显微镜可以观察到边缘带完整或解离成由平行微管形成的几个致密环，这些微管几乎沿着或伴随基质边缘分布。温度和秋水仙碱会导致微管解聚。在球形红细胞的基质中可以观察到致密的周边褶皱，但从未发现微管。因此，有人认为边缘带对于维持陆龟红细胞的椭圆形形状至关重要。
