Guo Baoyan, Chen Fengqiong, Liu Guo, Li Wentao, Li Wei, Zhuang Jianle, Zhang Xuejie, Wang Lashuang, Lei Bingfu, Hu Chaofan, Liu Yingliang
Key Laboratory for Biobased Materials and Energy of Ministry of Education/Guangdong Provincial Engineering Technology Research Center for Optical Agriculture, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Food Chem X. 2024 May 1;22:101422. doi: 10.1016/j.fochx.2024.101422. eCollection 2024 Jun 30.
Carbon dots (CDs) with different structures were prepared by electrolysis (PE-CDs) and hydrothermal (PH-CDs) methods using proanthocyanidins as precursors. The smaller size and lower zeta potential enabled the PE-CDs treated rice seedlings to exhibit greater resistance to salt stress. The fresh weight of rice seedlings under salt stress was significantly increased by spraying CDs every other day for two weeks. PE-CDs treated group exhibited a faster electron transport rate, and the SOD activity and flavonoid content were 2.5-fold and 0.23-fold higher than those of the salt stress-treated group. Furthermore, the metabolomics and transcriptomics analysis revealed that the PsaC gene of photosystem I was significantly up-regulated under PE-CDs treatment, which accelerated electron transfer in photosystem I. The up-regulation of BX1 and IGL genes encoding indole synthesis allowed rice to enhance stress tolerance through tryptophan and benzoxazine biosynthesis pathways. These findings offer help in purposefully synthesizing CDs and boosting food production.
以原花青素为前体，通过电解法（PE-CDs）和水热法（PH-CDs）制备了具有不同结构的碳点。较小的尺寸和较低的ζ电位使经PE-CDs处理的水稻幼苗对盐胁迫表现出更强的抗性。通过每隔一天喷洒碳点持续两周，盐胁迫下水稻幼苗的鲜重显著增加。PE-CDs处理组表现出更快的电子传递速率，超氧化物歧化酶（SOD）活性和类黄酮含量分别比盐胁迫处理组高2.5倍和0.23倍。此外，代谢组学和转录组学分析表明，在PE-CDs处理下，光系统I的PsaC基因显著上调，加速了光系统I中的电子传递。编码吲哚合成的BX1和IGL基因的上调使水稻能够通过色氨酸和苯并恶嗪生物合成途径增强胁迫耐受性。这些发现为有目的地合成碳点和提高粮食产量提供了帮助。
