Zhou Longhua, Zong Yingjie, Li Luli, Wu Shujun, Duan Mingming, Lu Ruiju, Liu Chenghong, Chen Zhiwei
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
Crop Breeding & Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
Front Plant Sci. 2022 Sep 14;13:961445. doi: 10.3389/fpls.2022.961445. eCollection 2022.
Salt stress is a major abiotic stress that threatens global rice production. It is particularly important to improve salt tolerance in upland rice because of its growth environment. Upland rice landrace 17SM-19 with high salt tolerance was obtained from a previous study. In this study, an integrated analysis of transcriptome and metabolome was performed to determine the responses of the rice seedling to salt stress. When treated with 100 mm NaCl, the rice seedling growth was significantly inhibited at 5 d, with inhibition first observed in shoot dry weight (SDW). Changes in potassium (K) content were associated with changes in SDW. In omics analyses, 1,900 differentially expressed genes (DEGs) and 659 differentially abundant metabolites (DAMs) were identified at 3 d after salt stress (DAS), and 1,738 DEGs and 657 DAMs were identified at 5 DAS. Correlation analyses between DEGs and DAMs were also conducted. The results collectively indicate that salt tolerance of upland rice landrace 17SM-19 seedlings involves many molecular mechanisms, such as those involved with osmotic regulation, ion balance, and scavenging of reactive oxygen species.
盐胁迫是威胁全球水稻生产的主要非生物胁迫。由于旱稻的生长环境，提高其耐盐性尤为重要。先前的一项研究获得了具有高耐盐性的旱稻地方品种17SM-19。在本研究中，进行了转录组和代谢组的综合分析，以确定水稻幼苗对盐胁迫的反应。用100 mM NaCl处理时，水稻幼苗生长在5天时受到显著抑制，首先观察到地上部干重（SDW）受到抑制。钾（K）含量的变化与SDW的变化相关。在组学分析中，在盐胁迫后3天（DAS）鉴定出1900个差异表达基因（DEG）和659个差异丰富代谢物（DAM），在5 DAS时鉴定出1738个DEG和657个DAM。还进行了DEG和DAM之间的相关性分析。结果共同表明，旱稻地方品种17SM-19幼苗的耐盐性涉及许多分子机制，如渗透调节、离子平衡和活性氧清除等机制。
