Wei Lijun, Cai Mengdie, Zhou Bixiao, Yuan Ping, Zhang Guowen, Cheng Xianglei
State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, PR China.
Jiangxi Province Key Laboratory of Disease Prevention and Public Health, School of Public Health, Jiangxi Medical College, Nanchang University, Nanchang 330006, PR China.
Food Chem X. 2024 Oct 5;24:101879. doi: 10.1016/j.fochx.2024.101879. eCollection 2024 Dec 30.
Thiram (THI) and asomate (ASO) are frequently detected agrochemicals in food samples due to widespread use as fungicides. However, their detection suffers from matrix interference, or the necessity of derivatization before measurement. Integrating HPLC with a post-column carbon dots (CDs)-enhanced chemiluminescence (CL) system makes it possible to simultaneously separate and selectively detect ASO and THI in fruit samples free of derivatization and deep purification. The studies on mechanisms demonstrated that CD intermediates of anionic radical were responsible for the remarkable CL enhancement of ASO or THI. The CL intensity is directly proportional to the fungicide concentration, with the ranges of linearity for ASO and THI being 5-250 μg·L ( ) and 4-200 μg·L ( ), respectively, and the corresponding limits of detection (S/N = 3) being 1.61 and 1.33 μg·L, respectively. The developed method enables the exploration of the novelly sulphur-targeted CL application based on carbon nanomaterials.
福美双（THI）和福美锌（ASO）作为常用的杀菌剂，在食品样本中经常被检测到。然而，它们的检测受到基质干扰，或者在测量前需要进行衍生化处理。将高效液相色谱（HPLC）与柱后碳点（CDs）增强化学发光（CL）系统相结合，使得在无需衍生化和深度纯化的情况下，能够同时分离并选择性检测水果样本中的ASO和THI。对作用机制的研究表明，阴离子自由基的CD中间体是ASO或THI显著增强CL的原因。CL强度与杀菌剂浓度成正比，ASO和THI的线性范围分别为5 - 250 μg·L（ ）和4 - 200 μg·L（ ），相应的检测限（S/N = 3）分别为1.61和1.33 μg·L。所开发的方法能够探索基于碳纳米材料的新型硫靶向CL应用。
