Zatz M M, Oliver J, Sztein M B, Skotnicki A B, Goldstein A L
J Biol Response Mod. 1985 Aug;4(4):365-76.
Thymosin fraction 5 (TF5) enhances interleukin-2 (IL-2) production by human peripheral blood mononuclear leukocytes (PBL) when cocultured in the presence of phytohemagglutinin (PHA). This important biological activity of TF5 is not due to the presence of two previously well-characterized component peptides, thymosin alpha 1 and thymosin beta 4, but rather to a new, still to be identified component of TF5. Comparison of TF5 with other well-defined thymic preparations and peptides indicates that this biological activity is unique to TF5 and a closely related extract prepared from porcine thymus tissue. Using a standard human PBL cell population obtained by leukopheresis and cryopreservation, it should now be possible to establish a reproducible bioassay for the isolation and characterization of one or more components of TF5 with the property of enhancing PHA-induced IL-2 production by human PBL.
胸腺素组分5（TF5）在有植物血凝素（PHA）存在的情况下与人外周血单个核白细胞（PBL）共培养时，可增强白细胞介素-2（IL-2）的产生。TF5的这一重要生物学活性并非归因于两种先前已充分表征的组分肽，即胸腺素α1和胸腺素β4，而是归因于TF5中一种新的、尚未确定的组分。TF5与其他明确的胸腺制剂和肽的比较表明，这种生物学活性是TF5以及从猪胸腺组织制备的一种密切相关提取物所特有的。使用通过白细胞分离术和冷冻保存获得的标准人PBL细胞群体，现在应该能够建立一种可重复的生物测定方法，用于分离和表征TF5中一种或多种具有增强人PBL的PHA诱导的IL-2产生特性的组分。
