Coastal Marine Field Station, School of Science, University of Waikato, Tauranga, New Zealand; Environmental Research Institute, The University of Waikato, Tauranga, New Zealand.
Coastal Marine Field Station, School of Science, University of Waikato, Tauranga, New Zealand; Environmental Research Institute, The University of Waikato, Tauranga, New Zealand.
Bioresour Technol. 2024 Jul;403:130850. doi: 10.1016/j.biortech.2024.130850. Epub 2024 May 16.
A practical two-product cascading biorefinery was developed to extract a biostimulant and cellulose from the freshwater filamentous macroalga Oedogonium calcareum grown while treating primary wastewater. Biostimulant production provides a valuable extract with production of disinfected residual biomass for further product development. Both Escherichia coli and F-specific RNA bacteriophage, indicators of human pathogens contamination, were absent from the residual biomass. The chemical composition of the biostimulant was complex, consisting of growth-promoting substances, free amino acids, and minerals. The O. calcareum cellulose fractions yielded between 9.5% and 10.1% (w/w) with purities from 84% to 90% and closely resembled microcrystalline cellulose. Biostimulant extraction improved cellulose quality by increasing crystallinity from 59% to 62%. Biomass condition, drying process, and biostimulant production influenced the crystallinity index. This study demonstrates a two-step process of biostimulant and cellulose extraction from wastewater-grown Oedogonium, simultaneously disinfecting biomass and isolating high-quality cellulose as a sustainable alternative to conventional extraction methods.
开发了一种实用的双产品级联生物炼制工艺，从淡水丝状大型藻类石灰藻中提取生物刺激素和纤维素，同时处理原废水。生物刺激素的生产提供了有价值的提取物，同时产生消毒后的剩余生物质，用于进一步的产品开发。残留生物质中均未检出大肠杆菌和 F 型 RNA 噬菌体，这两种物质是人病原体污染的指示物。生物刺激素的化学成分复杂，包含促进生长的物质、游离氨基酸和矿物质。石灰藻纤维素的提取率为 9.5%至 10.1%（w/w），纯度为 84%至 90%，与微晶纤维素非常相似。生物刺激素的提取通过将结晶度从 59%提高到 62%来改善纤维素的质量。生物质条件、干燥过程和生物刺激素的生产都会影响结晶度指数。本研究展示了一种从废水培养的石灰藻中提取生物刺激素和纤维素的两步法，同时对生物质进行消毒，并分离出高质量的纤维素，作为传统提取方法的可持续替代方法。
