Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, 21934, Alexandria, Egypt.
Microbial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
Sci Rep. 2024 May 17;14(1):11336. doi: 10.1038/s41598-024-59702-3.
Chitosan is a natural non-toxic, biocompatible, biodegradable, and mucoadhesive polymer. It also has a broad spectrum of applications such as agriculture, medical fields, cosmetics and food industries. In this investigation, chitosan nanoparticles were produced by an aqueous extract of Cympopogon citratus leaves as a reducing agent. According to the SEM and TEM micrographs, CNPs had a spherical shape, and size ranging from 8.08 to 12.01 nm. CNPs have a positively charged surface with a Zeta potential of + 26 mV. The crystalline feature of CNPs is determined by X-ray diffraction. There are many functional groups, including C꞊C, CH-OH, C-O, C-S, N-H, CN, CH and OH were detected by FTIR analysis. As shown by the thermogravimetric study, CNPs have a high thermal stability. For the optimization of the green synthesis of CNPs, a Face centered central composite design (FCCCD) with 30 trials was used. The maximum yield of CNPs (13.99 mg CNPs/mL) was produced with chitosan concentration 1.5%, pH 4.5 at 40 °C, and incubation period of 30 min. The antifungal activity of CNPs was evaluated against phytopathogenic fungus; Fusarium culmorum. A 100% rate of mycelial growth inhibition was gained by the application of 20 mg CNPs/mL. The antitumor activity of the green synthesized CNPs was examined using 6 different cell lines, the viability of the cells reduced when the concentration of green synthesized CNPs increased, the IC dose of the green synthesized CNPs on the examined cell lines HePG-2, MCF-7, HCT-116, PC-3, Hela and WI-38 was 36.25 ± 2.3, 31.21 ± 2.2, 67.45 ± 3.5, 56.30 ± 3.3, 44.62 ± 2.6 and 74.90 ± 3.8; respectively.
壳聚糖是一种天然的无毒、生物相容、可生物降解和具有黏膜黏附性的聚合物。它还具有广泛的应用，如农业、医疗领域、化妆品和食品工业。在这项研究中，壳聚糖纳米粒子是由香茅草叶的水提物作为还原剂生产的。根据 SEM 和 TEM 显微照片，CNPs 具有球形形状，尺寸范围为 8.08 至 12.01nm。CNPs 具有带正电荷的表面，Zeta 电位为+26mV。CNPs 的结晶特征由 X 射线衍射确定。通过傅里叶变换红外光谱分析检测到许多官能团，包括 C꞊C、CH-OH、C-O、C-S、N-H、CN、CH 和 OH。热重研究表明，CNPs 具有高热稳定性。为了优化 CNPs 的绿色合成，使用了 30 次的面心立方中心复合设计（FCCCD）。在壳聚糖浓度为 1.5%、pH 值为 4.5、孵育时间为 30 分钟的条件下，CNPs 的最大产率（13.99mg CNPs/mL）。CNPs 的抗真菌活性评估了对植物病原菌；尖孢镰刀菌。当应用 20mg CNPs/mL 时，获得了 100%的菌丝生长抑制率。用 6 种不同的细胞系检测了绿色合成的 CNPs 的抗肿瘤活性，随着绿色合成的 CNPs 浓度的增加，细胞的活力降低，绿色合成的 CNPs 对所检查的细胞系 HePG-2、MCF-7、HCT-116、PC-3、Hela 和 WI-38 的 IC 剂量分别为 36.25±2.3、31.21±2.2、67.45±3.5、56.30±3.3、44.62±2.6 和 74.90±3.8。
