即时人乳浓度的被动渗透法检测：新鲜人乳样本的综合分析。
Point-of-care human milk concentration by passive osmosis: comprehensive analysis of fresh human milk samples.
机构信息
Mother's Milk is Best Inc., R&D, 100 Business Park Drive, Unit #5, Tyngsboro, MA, 01879, USA.
Perinatal Institute, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH, 45229, USA.
出版信息
J Perinatol. 2024 Nov;44(11):1575-1583. doi: 10.1038/s41372-024-01988-2. Epub 2024 May 17.
OBJECTIVE
Preterm infants need enrichment of human milk (HM) for optimal growth. This study evaluated a novel, point-of-care human milk concentration (HMC) process for water removal from fresh HM samples by passive osmotic concentration.
STUDY DESIGN
Nineteen fresh HM samples were concentrated by incubation with the HMC devices for 3 h at 4 °C. Pre- and post-concentration HM samples were compared by HM properties for: pH, osmolality, macronutrients, enzyme activity, bioactive, and total cell viability.
RESULTS
Passive osmotic concentration reduced HM volume by an average of 16.3% ± 3.8% without a significant effect on pH or cell viability. Ten of the 41 HM components did not differ significantly (p > 0.05) between pre- and post-concentration samples. Twenty-three increased within the expected range by volume reduction. Six increased more than expected, two less than expected, and none decreased significantly.
CONCLUSION
Passive osmotic concentration of fresh HM can concentrate HM components by selective removal of water. HM osmolality and pH remained within neonatal feeding parameters.
目的
早产儿需要人乳（HM）的强化以实现最佳生长。本研究评估了一种新颖的、即时的人乳浓缩（HMC）方法，通过被动渗透浓缩从新鲜 HM 样本中去除水分。
研究设计
将 19 份新鲜 HM 样本在 4°C 下用 HMC 设备孵育 3 小时进行浓缩。通过 HM 特性比较浓缩前后 HM 样本：pH 值、渗透压、宏量营养素、酶活性、生物活性和总细胞活力。
结果
被动渗透浓缩平均减少了 16.3%±3.8%的 HM 体积，而 pH 值或细胞活力没有显著变化。41 种 HM 成分中有 10 种在浓缩前后样本之间没有显著差异（p＞0.05）。23 种成分按预期体积减少范围内增加。6 种成分增加超过预期，2 种成分减少低于预期，没有成分显著减少。
结论
新鲜 HM 的被动渗透浓缩可以通过选择性去除水分浓缩 HM 成分。HM 的渗透压和 pH 值仍在新生儿喂养参数范围内。
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