Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-Ro, Gangnam-Gu, Seoul, 06351, Republic of Korea.
Division of Allergy, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
BMC Microbiol. 2024 May 18;24(1):172. doi: 10.1186/s12866-024-03308-2.
We evaluated whether the sputum bacterial microbiome differs between nontuberculous mycobacteria pulmonary disease (NTM-PD) patients with stable disease not requiring antibiotic treatment and those requiring antibiotics.
We collected sputum samples from 21 clinically stable NTM-PD patients (stable group) and 14 NTM-PD patients needing antibiotic treatment (treatment group). We also obtained 13 follow-up samples from the stable group. We analyzed the 48 samples using 16S rRNA gene sequencing (V3-V4 region) and compared the groups.
In the linear discriminant analysis effect size (LEfSe) analysis, the species Porphyromonas pasteri, Haemophilus parahaemolyticus, Prevotella nanceiensis, and Gemella haemolysans were significantly more prevalent in the sputum of the stable group compared to the treatment group. No taxa showed significant differences in alpha-/beta-diversity or LEfSe between the 21 baseline and 13 follow-up sputum samples in the stable group. In the stable group, the genus Bergeyella and species Prevotella oris were less common in patients who achieved spontaneous culture conversion (n = 9) compared to those with persistent NTM positivity (n = 12) (effect size 3.04, p = 0.039 for Bergeyella; effect size 3.64, p = 0.033 for P. oris). In the treatment group, H. parainfluenzae was more common in patients with treatment success (n = 7) than in treatment-refractory patients (n = 7) (effect size 4.74, p = 0.013).
Our study identified distinct bacterial taxa in the sputum of NTM-PD patients based on disease status. These results suggest the presence of a microbial environment that helps maintain disease stability.
我们评估了病情稳定无需抗生素治疗的非结核分枝杆菌肺病（NTM-PD）患者与需要抗生素治疗的患者之间，其痰液细菌微生物组是否存在差异。
我们收集了 21 例临床稳定的 NTM-PD 患者（稳定组）和 14 例需要抗生素治疗的 NTM-PD 患者（治疗组）的痰液样本。我们还从稳定组获得了 13 份随访样本。我们使用 16S rRNA 基因测序（V3-V4 区）对 48 份样本进行了分析，并对两组进行了比较。
在线性判别分析效应量（LEfSe）分析中，与治疗组相比，物种牙龈卟啉单胞菌、副溶血嗜血杆菌、奈氏普雷沃菌和嗜血杆菌在稳定组的痰液中更为普遍。在稳定组的 21 份基线和 13 份随访痰液样本中，在 alpha-/beta-多样性或 LEfSe 方面没有观察到组间有显著差异。在稳定组中，与持续分枝杆菌阳性（n=12）的患者相比，自发培养转换（n=9）的患者中伯格氏菌属和普雷沃氏菌属或属更少（伯格氏菌属效应大小 3.04，p=0.039；普雷沃氏菌属效应大小 3.64，p=0.033）。在治疗组中，治疗成功（n=7）的患者比治疗失败（n=7）的患者中更常见副流感嗜血杆菌（效应大小 4.74，p=0.013）。
我们的研究根据疾病状况确定了 NTM-PD 患者痰液中的独特细菌分类群。这些结果表明存在维持疾病稳定的微生物环境。
