Scalia F, Arango V, Singman E L
Brain Res. 1985 Oct 7;344(2):267-80. doi: 10.1016/0006-8993(85)90804-2.
After studying pathway selection in the brain of Rana pipiens during unilateral optic nerve regeneration, several frogs were allowed to survive for lengthy periods for use in the present investigation. Retina flat-mounts were prepared from both eyes at 42-50 weeks postoperation. In some cases, HRP was infiltrated into both optic nerves prior to sacrifice to assist in identifying retinal ganglion cells. All specimens showed reduced cell-densities in the ganglion cell layer of the eye that had sustained the nerve regeneration. In addition, many ganglion cells were displaced, abnormally, into the inner plexiform layer, and the normally-situated cells formed irregular bands and islands in some parts of the retina. Cell-counts showed an apparently time-related change in neuron number ranging from a loss of 41% compared with the unaffected eye at 42 weeks, to losses as great as 71% at 50 weeks. The probable number of displaced amacrine cells in the ganglion cell layer, assumed to be unaffected by the experiment, was estimated at a maximum of 16%. Possible factors underlying the loss and displacement of ganglion cells are discussed.
在研究北美牛蛙单侧视神经再生过程中大脑中的通路选择后，留下几只青蛙存活较长时间以供本研究使用。在术后42 - 50周从双眼制备视网膜平铺标本。在某些情况下，在处死前将辣根过氧化物酶（HRP）注入双侧视神经以协助识别视网膜神经节细胞。所有标本均显示，经历神经再生的眼睛的神经节细胞层细胞密度降低。此外，许多神经节细胞异常地移位到内网状层，并且正常位置的细胞在视网膜的某些部位形成不规则的条带和岛状。细胞计数显示神经元数量呈现明显的时间相关变化，从42周时与未受影响的眼睛相比减少41%，到50周时减少高达71%。假定不受实验影响的神经节细胞层中移位无长突细胞的可能数量估计最多为16%。文中讨论了神经节细胞损失和移位的潜在可能因素。
