Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa, 16059, Turkey.
Department of Molecular Biology and Genetics, Faculty of Arts and Science, Yildiz Technical University, Istanbul, 34220, Turkey.
Syst Appl Microbiol. 2024 Jul;47(4):126518. doi: 10.1016/j.syapm.2024.126518. Epub 2024 May 14.
Eight isolates were obtained through a study on culture-dependent bacteria from fish farms and identified as members of the genus Flavobacterium based on pairwise analysis of the 16S rRNA gene sequences. The highest pairwise identity values were calculated as 98.8 % for strain F-30 and Flavobacterium bizetiae, 99.0 % for strain F-65 and Flavobacterium branchiarum, 98.7 % for strain F-126 and Flavobacterium tructae, 98.2 % for strain F-323 and Flavobacterium cupreum while 99.7 % identity level was detected for strain F-70 and Flavobacterium geliluteum. In addition, strains F-33, Fl-77, and F-70 shared 100 % identical 16S rRNA genes, while strains F-323 and Fl-318 showed 99.9 % identity. A polyphasic approach including comparative analysis of whole-genome data was employed to ascertain the taxonomic provenance of the strains. In addition to the morphological, physiological, biochemical and chemotaxonomic characteristics of the strains, the overall genome-relatedness indices of dDDH and ANI below the established thresholds confirmed the classification of the strains as five novel species within the genus Flavobacterium. The comprehensive genome analyses of the strains were also conducted to determine the biosynthetic gene clusters, virulence features and ecological distribution patterns. Based on the polyphasic characterisations, including comparative genome analyses, it is concluded that strains F-30 , F-65 , F-70 , F126 and F-323 represent five novel species within the genus Flavobacterium for which Flavobacterium piscisymbiosum sp. nov. F-30 (=JCM 34194 = KCTC 82254 ), Flavobacterium pisciphilum sp. nov. F-65 (=JCM 34197 = KCTC 82257 ), Flavobacterium flavipigmentatum sp. nov. F-70 (Fl-33 = Fl-77 = JCM 34198 = KCTC 82258 ), Flavobacterium lipolyticum sp. nov. F-126 (JCM 34199 = KCTC 82259 ) and Flavobacterium cupriresistens sp. nov. F-323 (Fl-318 = JCM 34200 = KCTC 82260 ), are proposed.
通过对鱼类养殖场依赖培养的细菌进行研究，获得了 8 个分离株，并根据 16S rRNA 基因序列的成对分析鉴定为黄杆菌属的成员。最高的成对身份值计算为菌株 F-30 和 Flavobacterium bizetiae 的 98.8%，菌株 F-65 和 Flavobacterium branchiarum 的 99.0%，菌株 F-126 和 Flavobacterium tructae 的 98.7%，菌株 F-323 和 Flavobacterium cupreum 的 98.2%，而菌株 F-70 和 Flavobacterium geliluteum 的身份水平检测到 99.7%。此外，菌株 F-33、Fl-77 和 F-70 共享 100%相同的 16S rRNA 基因，而菌株 F-323 和 Fl-318 显示出 99.9%的同一性。采用包括全基因组数据比较分析在内的多相方法来确定菌株的分类地位。除了菌株的形态、生理、生化和化学分类特征外，低于既定阈值的 dDDH 和 ANI 的全基因组相关性指数也证实了菌株被分类为黄杆菌属的五个新种。还对菌株进行了综合基因组分析，以确定生物合成基因簇、毒力特征和生态分布模式。基于多相特征，包括比较基因组分析，得出结论，菌株 F-30、F-65、F-70、F126 和 F-323 代表黄杆菌属的五个新种，其中 Flavobacterium piscisymbiosum sp. nov. F-30（=JCM 34194=KCTC 82254）、Flavobacterium pisciphilum sp. nov. F-65（=JCM 34197=KCTC 82257）、Flavobacterium flavipigmentatum sp. nov. F-70（Fl-33=Fl-77=JCM 34198=KCTC 82258）、Flavobacterium lipolyticum sp. nov. F-126（JCM 34199=KCTC 82259）和 Flavobacterium cupriresistens sp. nov. F-323（Fl-318=JCM 34200=KCTC 82260），被提议。
