State Key Laboratory of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean University of China, No. 1299, Sansha Road, Qingdao, Shandong Province, 266003, China.
Qingdao Institute of Marine Bioresources for Nutrition & Health Innovation, China.
Talanta. 2024 Aug 15;276:126259. doi: 10.1016/j.talanta.2024.126259. Epub 2024 May 14.
Hypoxanthine is a promising index for evaluating the freshness of various aquatic products. Combined the hydrogels containing upconversion nanoparticles (UCNPs), CoO NPs, and N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt/4-amino-antipyrine (TOPS/4-AAP) with a smartphone, a portable sensor was developed for the convenient, sensitive detection of hypoxanthine. With the HO from xanthine oxidase (XOD)-catalyzed reactions of hypoxanthine, the fluorescence of UCNPs was effectively quenched by the purple product produced from the oxidization of TOPS/4-AAP catalyzed by CoO NPs exhibiting peroxidase activity, among which the color change could be transformed into digital signals for quantification of hypoxanthine. The Green value in the RGB analysis of the fluorescence image was negatively proportional to hypoxanthine concentration in the range of 2.5-20 mg/L with a detection limit of 0.69 mg/L and a quantitation limit of 2.30 mg/L. Finally, this sensor was applied for hypoxanthine detection in real aquatic products, showing potential application for freshness evaluation of aquatic products.
次黄嘌呤是评估各种水产品新鲜度的有前途的指标。将含有上转换纳米粒子（UCNPs）、CoO NPs 和 N-乙基-N-（3-磺丙基）-3-甲基苯胺钠盐/4-氨基安替比林（TOPS/4-AAP）的水凝胶与智能手机结合，开发了一种便携式传感器，用于方便、灵敏地检测次黄嘌呤。通过黄嘌呤氧化酶（XOD）催化次黄嘌呤反应产生的 HO，TOPS/4-AAP 被 CoO NPs 催化氧化产生的紫色产物有效地猝灭了 UCNPs 的荧光，表现出过氧化物酶活性，其中颜色变化可以转化为数字信号，用于定量次黄嘌呤。荧光图像的 RGB 分析中的 Green 值与 2.5-20 mg/L 范围内的次黄嘌呤浓度成反比，检测限为 0.69 mg/L，定量限为 2.30 mg/L。最后，该传感器用于实际水产品中的次黄嘌呤检测，显示出在水产品新鲜度评估方面的潜在应用。
