Chair of Food Science, Institute of Nutritional Science, and TransMIT Center for Effect-Directed Analysis, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
Food Chem. 2024 Sep 30;453:139593. doi: 10.1016/j.foodchem.2024.139593. Epub 2024 May 11.
Ergot alkaloids, naturally occurring mycotoxins of Claviceps fungi, pose health risks. This necessitates accurate analysis methods to ensure food safety. This study explored the open-source miniaturized all-in-one 2LabsToGo system to analyze ergot alkaloids in whole rye samples. It is suited for sustainable atline analysis as it combines all planar chromatography tasks, allowing low-cost quality control in milling plants. The LOD and LOQ of ergocristine were determined to be 0.4 and 1.2 ng/zone, respectively. Detectability of ergot alkaloids was proven to be below the current maximum limit of 500 µg/kg for rye milling products. The repeatability (%RSD) was 4.1 % and the coefficient of determination of the analytical response (R) was 0.9918 for ergocristine. The mean recovery rate of ergot alkaloids in spiked whole rye grain was close to 100 %. Results of screening whole rye for ergot alkaloids were successfully verified by comparison with those obtained by conventional status quo HPTLC instrumentation.
麦角生物碱是一种天然存在的麦角真菌真菌毒素，对健康有危害。这就需要准确的分析方法来确保食品安全。本研究探索了开源的小型一体化 2LabsToGo 系统，以分析整个黑麦样品中的麦角生物碱。它非常适合可持续的现场分析，因为它结合了所有平面色谱任务，允许在制粉厂进行低成本的质量控制。确定了麦角柯宁的 LOD 和 LOQ 分别为 0.4 和 1.2 ng/zone。证明麦角生物碱的检测能力低于当前黑麦制粉产品 500 µg/kg 的最大限量。麦角柯宁的重复性（%RSD）为 4.1%，分析响应的确定系数（R）为 0.9918。添加到整个黑麦谷物中的麦角生物碱的平均回收率接近 100%。通过与传统现状 HPTLC 仪器获得的结果进行比较，成功验证了整个黑麦中麦角生物碱的筛选结果。
Zotero 插件