Pryjma J, Pituch-Noworolska A, Ruggiero I, Zembala M
Clin Immunol Immunopathol. 1985 Nov;37(2):245-52. doi: 10.1016/0090-1229(85)90156-4.
FcR+ and FcR- monocyte subsets were added to the pokeweed mitogen (PWM) or Staphylococcus aureus Cowan I-stimulated cultures of peripheral blood mononuclear cells (PBMC) or to PBMC depleted of monocytes. The numbers of immunoglobulin-secreting cells (ISC) and cells with intracytoplasmic immunoglobulins (PC) were evaluated 6 days later. The addition of FcR- subset increased the number of ISC in cultures of PBMC stimulated with PWM and reconstituted the response of monocyte depleted PBMC. In contrast, FcR+ monocytes suppressed PWM-induced response and, when added in high dose, also that induced by S. aureus. The FcR+ monocytes suppressed the response by inhibition of immunoglobulin secretion but not the development of PC. This suggests that FcR+ monocytes may modulate humoral response by preferential inhibition of the final differentiation of B lymphocytes into ISC.
将FcR+和FcR-单核细胞亚群添加到美洲商陆丝裂原（PWM）或金黄色葡萄球菌考恩I刺激的外周血单个核细胞（PBMC）培养物中，或添加到去除单核细胞的PBMC中。6天后评估免疫球蛋白分泌细胞（ISC）和胞浆内有免疫球蛋白的细胞（PC）的数量。添加FcR-亚群可增加PWM刺激的PBMC培养物中ISC的数量，并重建单核细胞去除的PBMC的反应。相反，FcR+单核细胞抑制PWM诱导的反应，当高剂量添加时，也抑制金黄色葡萄球菌诱导的反应。FcR+单核细胞通过抑制免疫球蛋白分泌来抑制反应，但不抑制PC的发育。这表明FcR+单核细胞可能通过优先抑制B淋巴细胞向ISC的最终分化来调节体液反应。
