China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China.
China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology (Shanghai), Shanghai Ocean University, Shanghai 201306, China; Center for Ecological Aquaculture (CEA), Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China.
Sci Total Environ. 2024 Aug 15;938:173233. doi: 10.1016/j.scitotenv.2024.173233. Epub 2024 May 17.
2-Methylisoborneol (2-MIB) is a common and widely distributed off-flavor compound in water. However, the toxic mechanisms of 2-MIB on aquatic organisms remain largely unexplored. In this study, grass carp larvae were exposed to different concentrations (0, 5, and 20 μg L) of 2-MIB for 96 h. The accumulation of 2-MIB in the dorsal muscle was measured. Histological analysis, ultrastructure observations, and transcriptomic sequencing were conducted on the liver tissues. The results showed that 2-MIB accumulated significantly in the fish muscle, with the accumulation increasing as the exposure concentration increased through gas chromatography-mass spectrometry (GC-MS) detection. Histological and ultrastructure observations indicated that 2-MIB caused concentration-dependent inflammatory infiltration and mitochondrial damage in the liver. Transcriptomic analysis revealed lipid metabolism disorders induced by exposure to 2-MIB in grass carp. Additionally, 5 μg L 2-MIB affected the neurodevelopment and cardiovascular system of grass carp larvae through extracellular matrix (ECM)-receptor interaction and focal adhesion pathway. Furthermore, several pathways related to the digestive system were significantly enriched, implying that 2-MIB may impact pancreatic secretion function, protein digestion and absorption processes. These findings provide new insights into the potential toxicological mechanisms of 2-MIB.
2-甲基异莰醇（2-MIB）是水中一种常见且广泛分布的异味化合物。然而，2-MIB 对水生生物的毒性机制在很大程度上仍未得到探索。在这项研究中，草鱼幼鱼暴露于不同浓度（0、5 和 20μg/L）的 2-MIB 中 96 小时。测量了背部肌肉中 2-MIB 的积累量。对肝脏组织进行了组织学分析、超微结构观察和转录组测序。结果表明，2-MIB 通过气相色谱-质谱（GC-MS）检测在鱼肌肉中显著积累，随着暴露浓度的增加而增加。组织学和超微结构观察表明，2-MIB 导致肝脏中炎症浸润和线粒体损伤呈浓度依赖性。转录组分析显示，草鱼暴露于 2-MIB 会导致脂质代谢紊乱。此外，5μg/L 的 2-MIB 通过细胞外基质（ECM）-受体相互作用和黏附斑途径影响草鱼幼鱼的神经发育和心血管系统。此外，与消化系统相关的几个途径显著富集，表明 2-MIB 可能影响胰腺分泌功能、蛋白质消化和吸收过程。这些发现为 2-MIB 的潜在毒理学机制提供了新的见解。
