Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, China.
People's Government of Xianliang Town, Qingyuan County, Zhejiang Province 323800, China.
Gene. 2024 Sep 25;923:148588. doi: 10.1016/j.gene.2024.148588. Epub 2024 May 18.
Polygalacturonase inhibitor protein (PGIP) restricts fungal growth and colonization and functions in plant immunity. Gray mold in cucumber is a common fungal disease caused by Botrytis cinerea, and is widespread and difficult to control in cucumber (Cucumis sativus L.) production. In this study, Cucumis sativus polygalacturonase-inhibiting protein 2 (CsPGIP2) was found to be upregulated in response to gray mold in cucumber. CsPGIP2 was detected in the endoplasmic reticulum, cell membrane, and cell wall after transient transformation of protoplasts and tobacco. A possible interaction between Botrytis cinerea polygalacturonase 3 (BcPG3) and CsPGIP2 was supported by protein interaction prediction and BiFC analysis. Transgenic Arabidopsis plants expressing CsPGIP2 were constructed and exhibited smaller areas of gray mold infection compared to wild type (WT) plants after simultaneous inoculation. Evans blue dye (EBD) confirmed greater damage for WT plants, with more intense dyeing than for the transgenic Arabidopsis. Interestingly, compared to WT, transgenic Arabidopsis exhibited higher superoxide dismutase (AtSOD1) expression, antioxidant enzyme activities, lignin content, net photosynthetic rate (Pn), and photochemical activity. Our results suggest that CsPGIP2 stimulates a variety of plant defense mechanisms to enhance transgenic Arabidopsis resistance against gray mold disease.
多聚半乳糖醛酸酶抑制剂蛋白 (PGIP) 限制真菌生长和定植，并在植物免疫中发挥作用。黄瓜灰霉病是由灰葡萄孢引起的一种常见真菌病，在黄瓜（Cucumis sativus L.）生产中广泛存在且难以控制。在这项研究中，发现黄瓜多聚半乳糖醛酸酶抑制蛋白 2（CsPGIP2）对黄瓜灰霉病有上调反应。在质体和烟草的瞬时转化后，CsPGIP2 检测到在内质网、细胞膜和细胞壁中。蛋白互作预测和 BiFC 分析支持灰葡萄孢多聚半乳糖醛酸酶 3（BcPG3）和 CsPGIP2 之间可能存在相互作用。构建了表达 CsPGIP2 的转基因拟南芥植物，与野生型（WT）植物相比，在同时接种后，其灰霉病感染面积更小。Evans 蓝染料（EBD）证实 WT 植物的损伤更大，染色比转基因拟南芥更强烈。有趣的是，与 WT 相比，转基因拟南芥表现出更高的超氧化物歧化酶（AtSOD1）表达、抗氧化酶活性、木质素含量、净光合速率（Pn）和光化学活性。我们的结果表明，CsPGIP2 刺激多种植物防御机制，增强转基因拟南芥对灰霉病的抗性。
