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采用 UPLC-Q-TOF/MS 联合网络药理学揭示柴胡加龙骨牡蛎汤治疗伴有焦虑障碍的眩晕的作用机制。

Combination of UPLC-Q-TOF/MS and network pharmacology to reveal the mechanism of Chaihu-jia-Longgu-Muli decoction for treating vertigo with anxiety disorder.

机构信息

Changchun University of Chinese Medicine, Changchun, Jilin, China.

Dizziness Clinic, Jilin Provincial Academy of Chinese Medicine Sciences, Changchun, Jilin, China.

出版信息

Biomed Chromatogr. 2024 Jul;38(7):e5881. doi: 10.1002/bmc.5881. Epub 2024 May 19.

Abstract

Chaihu-jia-Longgu-Muli decoction (CLMD) has been proven clinically effective in treating vertigo with anxiety disorder. However, the mechanism is not clear. This study aimed to explore the mechanism of CLMD in treating vertigo with anxiety disorder based on ultra-performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-Q-TOF/MS) and network pharmacology. UPLC-Q-TOF/MS was performed to identify the compounds in blood and the targets of compounds of CLMD in vertigo and anxiety were searched using databases. A protein-protein interaction network was built to screen the core targets. The core targets were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. In addition, the vertigo with anxiety rat model was used to verify the results. A total of 22 compounds were absorbed into the blood. Eighty-one potential targets associated with CLMD for vertigo with anxiety disorder were identified through network pharmacological analysis. Subsequently, GO and KEGG analysis showed that CLMD treatment for vertigo with anxiety disorder is associated with neurotransmitter levels and other pertinent physiological processes. The results of the animal experiments showed that CLMD decreased the levels of serotonin, norepinephrine and dopamine, alleviating the symptoms of vertigo and anxiety disorder in model rats. The study revealed CLMD could alleviate vertigo and anxiety symptoms through reducing the levels of neurotransmitters.

摘要

柴胡加龙骨牡蛎汤已被临床证实可有效治疗伴有焦虑症的眩晕。但其作用机制尚不清楚。本研究旨在通过超高效液相色谱-四级杆飞行时间/质谱联用(UPLC-Q-TOF/MS)和网络药理学方法,探讨柴胡加龙骨牡蛎汤治疗伴有焦虑症的眩晕的作用机制。采用 UPLC-Q-TOF/MS 技术对柴胡加龙骨牡蛎汤治疗眩晕的入血成分及复方中化合物的靶点进行鉴定,通过数据库检索寻找治疗眩晕伴焦虑症的复方化合物靶点。构建蛋白质-蛋白质相互作用网络,筛选核心靶点。采用基因本体(GO)和京都基因与基因组百科全书(KEGG)通路分析对核心靶点进行分析。此外,还建立了伴有焦虑症的眩晕大鼠模型来验证结果。研究共鉴定出 22 种进入血液的化合物,通过网络药理学分析,发现了 81 个与柴胡加龙骨牡蛎汤治疗眩晕伴焦虑症相关的潜在靶点。随后,GO 和 KEGG 分析表明,柴胡加龙骨牡蛎汤治疗眩晕伴焦虑症与神经递质水平和其他相关生理过程有关。动物实验结果表明,柴胡加龙骨牡蛎汤能降低模型大鼠血清中 5-羟色胺、去甲肾上腺素和多巴胺的水平,从而缓解眩晕和焦虑症状。该研究表明,柴胡加龙骨牡蛎汤可通过降低神经递质水平来缓解眩晕和焦虑症状。

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