Jirillo E, Antonaci S, Kiyono H, Michalek S M, McGhee J R
J Immunol. 1985 Nov;135(5):3473-8.
We have previously shown that Salmonella minnesota R345 (Rb) spontaneously binds to 50 to 55% of human peripheral blood mononuclear cells (PBMC). In the present study, we have compared Rb cytoadherence to lymphoid cells from various tissues of lipopolysaccharide (LPS) hyporesponsive (Lpsd) and LPS responsive (Lpsn) mouse strains. A higher number of spleen cells from Lpsd mice (C3H/HeJ and C57BL/10ScN) bound Rb bacteria (22 to 30%) than cells from Lpsn mice (4 to 9%). Rb bound mainly to T cells, and cytoadherence occurred in both Lyt-1+ and Lyt-2+ T cell subsets. By contrast, purified splenic B cells from Lpsd and Lpsn mice gave less than 4% Rb cytoadherence. In both mouse strains, cytoadherence was mediated by the homologous LPS structure, because purified Rb-LPS blocked Rb Salmonella binding to T cells. On the other hand, smooth Salmonella typhimurium LT-2 LPS (S-LPS) and Salmonella R595 (Re) LPS (Re-LPS), which contain mainly lipid A, were without effect on Rb binding. Increased Rb binding was seen with T cells from Peyer's patches (PP), mesenteric lymph nodes (MLN), and peripheral blood than from spleen of C3H/HeN (Lpsn) mice; however, greater cytoadherence was always seen with T cells of these tissues from C3H/HeJ mice. Interestingly, treatment of whole spleen or purified T cells from C3H/HeN mice with neuraminidase enhanced cytoadherence to levels seen with C3H/HeJ cells. The observed Rb binding to PP, MLN, and PBMC cells in both mouse strains suggests that gut microbial environment may play an important role in Rb cytoadherence. This is also supported by the evidence that when spleen cells of germfree and conventional mice were tested for Rb binding, higher cytoadherence was observed in conventional mice only. Taken together, these results indicate that T cells of Lpsd mice express binding site(s) for Salmonella, whereas Lpsn mice have T cells with these structure(s) in a cryptic configuration.
我们之前已经表明，明尼苏达沙门氏菌R345（Rb）能自发结合50%至55%的人外周血单个核细胞（PBMC）。在本研究中，我们比较了Rb对脂多糖（LPS）低反应性（Lpsd）和LPS反应性（Lpsn）小鼠品系不同组织中淋巴细胞的细胞黏附情况。来自Lpsd小鼠（C3H/HeJ和C57BL/10ScN）的脾脏细胞结合Rb细菌的数量（22%至30%）高于来自Lpsn小鼠的细胞（4%至9%）。Rb主要结合T细胞，细胞黏附发生在Lyt-1⁺和Lyt-2⁺ T细胞亚群中。相比之下，来自Lpsd和Lpsn小鼠的纯化脾脏B细胞的Rb细胞黏附率低于4%。在这两种小鼠品系中，细胞黏附由同源LPS结构介导，因为纯化的Rb-LPS可阻断Rb沙门氏菌与T细胞的结合。另一方面，主要含脂质A的光滑型鼠伤寒沙门氏菌LT-2 LPS（S-LPS）和沙门氏菌R595（Re）LPS（Re-LPS）对Rb结合无影响。与C3H/HeN（Lpsn）小鼠脾脏的T细胞相比，派尔集合淋巴结（PP）、肠系膜淋巴结（MLN）和外周血中的T细胞对Rb的结合增加；然而，C3H/HeJ小鼠这些组织中的T细胞的细胞黏附总是更高。有趣的是，用神经氨酸酶处理C3H/HeN小鼠的全脾或纯化T细胞可使细胞黏附增加至C3H/HeJ细胞的水平。在这两种小鼠品系中观察到的Rb与PP、MLN和PBMC细胞的结合表明，肠道微生物环境可能在Rb细胞黏附中起重要作用。无菌小鼠和普通小鼠的脾脏细胞进行Rb结合测试的证据也支持了这一点，即仅在普通小鼠中观察到更高的细胞黏附。综上所述，这些结果表明，Lpsd小鼠的T细胞表达沙门氏菌的结合位点，而Lpsn小鼠的T细胞具有呈隐蔽构型的这些结构。
