School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230022, China.
State Key Lab of Proteomics, National Center for Protein Sciences at Beijing, Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China.
Yi Chuan. 2023 Nov 20;45(11):950-962. doi: 10.16288/j.yczz.23-206.
The CRISPR genome editing technology shows great application prospects in gene manipulation and infectious disease research, and is of great value for effective control and cure of infectious diseases. It has been utilized to generate specific disease models in cells, organoids and animals, which provide great convenience for research into the molecular mechanisms associated with infectious diseases. CRISPR screening technology enables high-throughput identification of risk factors. New molecular diagnostic tools based on CRISPR offer a more sensitive and faster method for detecting pathogens. The use of CRISPR tools to introduce resistance genes or to specifically destroy risk genes and virus genomes is intended to help prevent or treat infectious diseases. This review discusses the application of CRISPR genome editing technologies in the construction of disease models, screening of risk factors, pathogen diagnosis, and prevention and treatment of infectious diseases, thereby providing a reference for follow-up research in pathogenesis, diagnosis, prevention and treatment of infectious diseases.
CRISPR 基因组编辑技术在基因操作和传染病研究中显示出巨大的应用前景，对于有效控制和治疗传染病具有重要价值。它已被用于在细胞、类器官和动物中产生特定的疾病模型，为研究与传染病相关的分子机制提供了极大的便利。CRISPR 筛选技术能够高通量地鉴定风险因素。基于 CRISPR 的新型分子诊断工具为检测病原体提供了更敏感和更快的方法。利用 CRISPR 工具引入抗性基因或专门破坏风险基因和病毒基因组，旨在帮助预防或治疗传染病。本综述讨论了 CRISPR 基因组编辑技术在疾病模型构建、风险因素筛选、病原体诊断以及传染病预防和治疗中的应用，为后续传染病发病机制、诊断、预防和治疗的研究提供参考。
