Foster P N, Heatley R V, Losowsky M S
J Clin Lab Immunol. 1985 Jun;17(2):75-7.
An imbalance in immunoregulatory cells might explain the increase in autoimmune phenomena associated with hyposplenism. Using the monoclonal antibodies OKT3, OKT4 and OKT8 to identify T-lymphocyte subsets, we have studied 19 patients with treated coeliac disease, 9 of whom had hyposplenism, 10 splenectomized subjects, 10 gastrointestinal control patients and 10 normal subjects. There was no significant difference in the proportion of lymphocytes, OKT3+, OKT4+ or OKT8+ cells, nor in the OKT4+/OKT8+ ratio, between any of the patient groups and normal or control subjects. Splenectomized subjects had higher total lymphocyte counts than normals (p less than 0.01). Hyposplenic coeliac patients had higher total lymphocyte counts than other coeliacs (p less than 0.05). Hyposplenic coeliac patients and splenectomized subjects tended to have an increase in the absolute number of OKT8+ cells, which in the latter group was statistically significant (p less than 0.05). We conclude that the increased frequency of autoimmune phenomena associated with hyposplenism due to coeliac disease or after splenectomy is not the result of an altered ratio of OKT4+/OKT8+ cells or a deficiency of OKT8+ cells.
免疫调节细胞的失衡可能解释了与脾功能减退相关的自身免疫现象的增加。我们使用单克隆抗体OKT3、OKT4和OKT8来识别T淋巴细胞亚群，研究了19例接受治疗的乳糜泻患者，其中9例有脾功能减退，10例脾切除患者，10例胃肠道对照患者和10例正常受试者。在任何患者组与正常或对照受试者之间，淋巴细胞、OKT3+、OKT4+或OKT8+细胞的比例以及OKT4+/OKT8+比值均无显著差异。脾切除患者的总淋巴细胞计数高于正常人（p<0.01）。脾功能减退的乳糜泻患者的总淋巴细胞计数高于其他乳糜泻患者（p<0.05）。脾功能减退的乳糜泻患者和脾切除患者的OKT8+细胞绝对数量有增加的趋势，在脾切除组中具有统计学意义（p<0.05）。我们得出结论，由于乳糜泻或脾切除术后导致的脾功能减退相关的自身免疫现象频率增加，不是OKT4+/OKT8+细胞比例改变或OKT8+细胞缺乏的结果。
