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含DEP结构域蛋白1作为肺腺癌预后不良的生物标志物。

DEP domain containing 1 as a biomarker for poor prognosis in lung adenocarcinoma.

作者信息

Li Cuixian, Zhu Xiaoling

机构信息

First Affiliated Hospital of Dali University, Dali, Yunnan, China.

出版信息

Heliyon. 2024 May 3;10(9):e30642. doi: 10.1016/j.heliyon.2024.e30642. eCollection 2024 May 15.

DOI:10.1016/j.heliyon.2024.e30642
PMID:38765113
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11101781/
Abstract

OBJECTIVE

The DEP domain-containing 1 (DEPDC1) gene is essential in the development and advancement of different types of cancer. This study is to examine the levels of DEPDC1 in lung adenocarcinoma (LUAD), and to determine its relationship with clinical results and immune response. The goal is to assess its potential as a biomarker and therapeutic target for LUAD.

METHODS

By comprehensively utilizing the Cancer Genome Atlas (TCGA), gene Expression Synthesis (GEO), UALCAN, cBioPortal, TISIDB databases and online platforms, we conducted a bioinformatics analysis to investigate DEPDC1 gene survival analysis, prognostic diagnosis, prognostic survival, immune cell infiltration, DNA methylation, and the correlation of genetic mutations in LUAD. The results were validated through cell assay and immunohistochemical staining.

RESULTS

DEPDC1 shows high levels of expression in the majority of tumors, with its expression being notably elevated in LUAD compablue to normal tissues. The expression of DEPDC1 varies based on the clinical characteristics of patients with LUAD. DEPDC1 expression affects the survival prognosis and prognostic model construction of LUAD patients. In addition, the presence of DEPDC1 is linked to immune infiltration. Various chemokines and chemokine receptors, immunoinhibitors and immune-stimulators in LUAD are significantly correlated with DEPDC1 methylation levels. Cell experiments confirmed through qPCR that the mRNA expression of DEPDC1 in LUAD was markedly elevated in comparison to the normal population, and immunohistochemistry showed positive DEPDC1 expression in LUAD pathological sections.

CONCLUSION

Systematic analysis and experiments have verified that DEPDC1 serves as a biomarker for detecting early, prediction of survival, and evaluation of immune cell infiltration in LUAD.

摘要

目的

含DEP结构域蛋白1（DEPDC1）基因在不同类型癌症的发生和发展中至关重要。本研究旨在检测肺腺癌（LUAD）中DEPDC1的水平，并确定其与临床结果和免疫反应的关系。目标是评估其作为LUAD生物标志物和治疗靶点的潜力。

方法

通过综合利用癌症基因组图谱（TCGA）、基因表达综合数据库（GEO）、UALCAN、cBioPortal、TISIDB数据库及在线平台，我们进行了生物信息学分析，以研究LUAD中DEPDC1基因的生存分析、预后诊断、预后生存、免疫细胞浸润、DNA甲基化及基因突变相关性。结果通过细胞实验和免疫组织化学染色进行验证。

结果

DEPDC1在大多数肿瘤中呈现高水平表达，在LUAD中的表达相较于正常组织显著升高。DEPDC1的表达因LUAD患者的临床特征而异。DEPDC1表达影响LUAD患者的生存预后和预后模型构建。此外，DEPDC1的存在与免疫浸润相关。LUAD中的各种趋化因子和趋化因子受体、免疫抑制剂和免疫刺激剂与DEPDC1甲基化水平显著相关。通过qPCR进行细胞实验证实，与正常人群相比，LUAD中DEPDC1的mRNA表达明显升高，免疫组织化学显示LUAD病理切片中DEPDC1表达呈阳性。

结论

系统分析和实验已证实，DEPDC1可作为检测LUAD早期、预测生存及评估免疫细胞浸润的生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/d42e6d5a7008/gr16.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/d42e6d5a7008/gr16.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/2aa900b5bbca/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/636d77565edd/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/be4c46ee4206/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/af7b39c17fe9/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/d28e4b8f89bf/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/b36d51349104/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/59dfde80296c/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/e3d4943ef49d/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/0c4f1808ff3a/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/a8fa099aee7b/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/178ccf19a3c4/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/3f42eb5472dc/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/4a3de15abf7c/gr13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/1181e36de570/gr14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/64e1a4c037e0/gr15.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/335e/11101781/d42e6d5a7008/gr16.jpg

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