Wei Qin, Miao Tianshu, Zhang Pengju, Jiang Baodong, Yan Hua
Department of Biochemistry and Molecular Biology, Shandong University School of Basic Medical Sciences, Jinan, China.
Department of Radiology, Qilu Hospital of Shandong University, Jinan, China.
Front Genet. 2022 Sep 9;13:984575. doi: 10.3389/fgene.2022.984575. eCollection 2022.
G Protein Subunit Gamma 7 (GNG7), an important regulator of cell proliferation and cell apoptosis, has been reported to be downregulated in a variety of tumors including lung adenocarcinoma (LUAD). However, the correlation between low expression of GNG7 and prognosis of LUAD as well as the immune infiltrates of LUAD remains unclear. The samples were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). R software was performed for statistical analysis. GNG7 expression and its prognostic value in LUAD were assessed through statistically analyzing the data from different databases. A nomogram was constructed to predict the impact of GNG7 on prognosis. Gene set enrichment analysis (GSEA) and single-sample gene set enrichment analyses GSEA (ssGSEA) were employed to determine the potential signal pathways and evaluated the immune cell infiltration regulated by GNG7. The prognostic significance of GNG7 expression associated with immune cell infiltration was investigated using the Tumor Immune Estimation Resource 2.0 (TIMER2.0) and the Kaplan-Meier plotter database. The UALCAN, cBio Cancer Genomics Portal (cBioPortal) and MethSurv database were used to analyze the correlation between the methylation of GNG7 and its mRNA expression as well as prognostic significance. GNG7 was demonstrated to be down-regulated in LUAD and its low expression was associated with poor prognosis. A clinical reliable prognostic-predictive model was constructed. Pathway enrichment showed that GNG7 was highly related to the B cell receptor signaling pathway. Further analysis showed that GNG7 was positively associated with B cell infiltration and low levels of B cell infiltration tended to associate with worse prognosis in patients with low GNG7 expression. Moreover, methylation analysis suggested hypermethylation may contribute to the low expression of GNG7 in LUAD. Decreased expression of GNG7 at least partly caused by hypermethylation of the GNG7 promoter is closely associated with poor prognosis and tumor immune cell infiltration (especially B cells) in LUAD. These results suggest that GNG7 may be a promising prognostic biomarker and a potential immunotherapeutic target for LUAD, which provides new insights into immunotherapy for LUAD.
G蛋白亚基γ7（GNG7）是细胞增殖和细胞凋亡的重要调节因子，据报道在包括肺腺癌（LUAD）在内的多种肿瘤中表达下调。然而，GNG7低表达与LUAD预后以及LUAD免疫浸润之间的相关性仍不清楚。样本取自癌症基因组图谱（TCGA）和基因表达综合数据库（GEO）。使用R软件进行统计分析。通过对来自不同数据库的数据进行统计分析，评估GNG7在LUAD中的表达及其预后价值。构建了一个列线图来预测GNG7对预后的影响。采用基因集富集分析（GSEA）和单样本基因集富集分析GSEA（ssGSEA）来确定潜在的信号通路，并评估GNG7调节的免疫细胞浸润情况。使用肿瘤免疫评估资源2.0（TIMER2.0）和Kaplan-Meier绘图仪数据库研究GNG7表达与免疫细胞浸润相关的预后意义。使用UALCAN、cbioportal癌症基因组学门户（cbioportal）和MethSurv数据库分析GNG7甲基化与其mRNA表达之间的相关性以及预后意义。结果表明，GNG７在LUAD中表达下调，其低表达与预后不良相关。构建了一个临床可靠的预后预测模型。通路富集分析表明，GNG7与B细胞受体信号通路高度相关。进一步分析表明，GNG7与B细胞浸润呈正相关
