Oyovwi Mega O, Ben-Azu Benneth, Tesi Edesiri P, Ojetola Abodunrin A, Olowe Temitope G, Joseph Uchechukwu G, Emojevwe Victor, Oghenetega Onome B, Rotu Rume A, Rotu Rotu A, Falajiki Faith Y
Department of Physiology, Adeleke University Ede, Osun State, Nigeria.
Department of Hunan Physiology, Achievers University Owo, Ondo State, Nigeria.
Int J Biochem Mol Biol. 2024 Apr 15;15(2):34-50. doi: 10.62347/ORPK5021. eCollection 2024.
Cancer chemotherapy with doxorubicin (DOX) has been linked to serious testicular damage and spermatotoxicity due to the induction of oxidative stress, inflammation, and apoptosis. Thus, the current study was carried out to assess the potential ameliorative impact of diosmin, an antioxidant drug, against DOX-mediated spermatoxicity and testicular injury in rats.
In the experimental protocol, rats were grouped into 4: Group 1 received vehicle and saline for 8 weeks while group 2 received diosmin and saline concomitantly for 8 weeks. Group 3 was given 3 mg/kg intraperitoneal DOX once every 7 days for 8 weeks. Group 4 was given 40 mg/kg of diosmin orally for 56 days followed by DOX diosmin administration after one hour. After 56 days of treatment, sperm quality, hormonal testing, biochemical parameters, and histological alterations in the testes were evaluated.
DOX-induced reduce spermatogenic function, testicular 3- and 17β-Hydroxysteroid dehydrogenases, and serum follicle stimulating hormone, luteinizing hormone, and testosterone. It also enhanced inflammation, testicular oxidative damage, and apoptosis. The histopathologic examinations corroborated the biochemical results obtained. Significantly, diosmin treatment reduced DOX-induced injury, as evidenced by restored testicular architecture, increased steroidogenesis, preservation of spermatogenesis, suppression of oxide-inflammatory response, and apoptosis.
It was found that through diosmin antioxidant, anti-apoptotic, and anti-oxido-inflammatory it presents a possible therapeutic alternative for protecting testicular tissue against DOX's harmful effects.
阿霉素（DOX）化疗与严重的睾丸损伤和精子毒性有关，这是由于氧化应激、炎症和细胞凋亡的诱导。因此，本研究旨在评估抗氧化药物地奥司明对DOX介导的大鼠精子毒性和睾丸损伤的潜在改善作用。
在实验方案中，大鼠被分为4组：第1组接受载体和生理盐水8周，而第2组同时接受地奥司明和生理盐水8周。第3组每7天腹腔注射3mg/kg DOX，共8周。第4组口服40mg/kg地奥司明56天，1小时后给予DOX和地奥司明。治疗56天后，评估精子质量、激素检测、生化参数和睾丸组织学改变。
DOX诱导精子发生功能降低、睾丸3-和17β-羟基类固醇脱氢酶降低，以及血清促卵泡激素、黄体生成素和睾酮降低。它还增强了炎症、睾丸氧化损伤和细胞凋亡。组织病理学检查证实了所获得的生化结果。值得注意的是，地奥司明治疗减少了DOX诱导的损伤，这通过恢复睾丸结构、增加类固醇生成、保护精子发生、抑制氧化炎症反应和细胞凋亡得到证明。
发现地奥司明通过其抗氧化、抗凋亡和抗氧化炎症作用，为保护睾丸组织免受DOX的有害影响提供了一种可能的治疗选择。
