全面分析 TBC1Ds 在人肝细胞癌中的致癌和预后作用。

A comprehensive analysis of the oncogenic and prognostic role of TBC1Ds in human hepatocellular carcinoma.

机构信息

The Affiliated Geriatric Hospital of Nanjing Medical University, Nanjing, China.

出版信息

PeerJ. 2024 May 14;12:e17362. doi: 10.7717/peerj.17362. eCollection 2024.

DOI:10.7717/peerj.17362

PMID:38766486

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11100476/

Abstract

BACKGROUNDS

TBC1D family members (TBC1Ds) are a group of proteins that contain the Tre2-Bub2-Cdc16 (TBC) domain. Recent studies have shown that TBC1Ds are involved in tumor growth, but no analysis has been done of expression patterns and prognostic values of TBC1Ds in hepatocellular carcinoma (HCC).

METHODS

The expression levels of TBC1Ds were evaluated in HCC using the TIMER, UALCN and Protein Atlas databases. The correlation between the mRNA levels of TBC1Ds and the prognosis of patients with HCC in the GEPIA database was then analyzed. An enrichment analysis then revealed genes that potentially interact with TBC1Ds. The correlation between levels of TBC1Ds and tumor-infiltrating immune cells (TIICs) in HCC were studied using the TIMER 2.0 database. Finally, a series of assays verified the role of TBC1Ds in HCC progression.

RESULTS

This study revealed the upregulated expression of TBC1Ds in HCC and the strong positive correlation between the mRNA levels of TBC1Ds and poor prognosis of patients with HCC. The functions of TBC1Ds were mainly related to autophagy and the AMPK pathway. There was also a significant correlation between level of TBC1Ds and tumor-infiltrating immune cells (TIICs) in HCC. The promoting role of TBC1Ds in HCC progression was verified assays.

CONCLUSION

The results of this analysis indicate that TBC1Ds may serve as new biomarkers for early diagnosis and treatment of HCC.

摘要

背景

TBC1D 家族成员（TBC1Ds）是一组含有 Tre2-Bub2-Cdc16（TBC）结构域的蛋白质。最近的研究表明，TBC1Ds 参与肿瘤生长，但尚未分析 TBC1Ds 在肝细胞癌（HCC）中的表达模式和预后价值。

方法

使用 TIMER、UALCN 和 Protein Atlas 数据库评估 HCC 中 TBC1D 的表达水平。然后分析 GEPIA 数据库中 TBC1D mRNA 水平与 HCC 患者预后的相关性。接着进行富集分析，揭示可能与 TBC1D 相互作用的基因。使用 TIMER 2.0 数据库研究 TBC1D 水平与 HCC 中肿瘤浸润免疫细胞（TIIC）的相关性。最后，一系列实验验证了 TBC1D 在 HCC 进展中的作用。

结果

本研究揭示了 HCC 中 TBC1D 的上调表达，以及 TBC1D mRNA 水平与 HCC 患者不良预后之间的强烈正相关。TBC1D 的功能主要与自噬和 AMPK 途径有关。TBC1D 水平与 HCC 中肿瘤浸润免疫细胞（TIIC）之间也存在显著相关性。实验验证了 TBC1D 在 HCC 进展中的促进作用。