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秀丽隐杆线虫对抗铜绿假单胞菌 PA14 感染的生存分析方案。

Protocol for survival assay of Caenorhabditis elegans to Pseudomonas aeruginosa PA14 infection.

机构信息

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, Hunan 410082, China.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, Hunan 410082, China.

出版信息

STAR Protoc. 2024 Jun 21;5(2):103070. doi: 10.1016/j.xpro.2024.103070. Epub 2024 May 19.

DOI:10.1016/j.xpro.2024.103070
PMID:38768031
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11111813/
Abstract

The nematode Caenorhabditis elegans is a powerful model organism for studying the molecular and cellular mechanisms of innate immunity governed by the intestine. Here, we present a protocol to perform C. elegans survival assays to infection by the bacterial pathogen Pseudomonas aeruginosa PA14. Specifically, we describe steps for preparing C. elegans strains and PA14 bacteria for survival assays. This protocol will assist researchers to study genes involved in intestinal innate immunity and gut defense against pathogen infection. For complete details on the use and execution of this protocol, please refer to Liu et al. and Zheng et al..

摘要

秀丽隐杆线虫是研究肠道固有免疫的分子和细胞机制的强大模式生物。本文提供了秀丽隐杆线虫抗细菌病原体铜绿假单胞菌 PA14 感染的生存分析实验方案。具体而言，我们描述了进行秀丽隐杆线虫菌株和 PA14 细菌生存分析实验的步骤。本方案将有助于研究人员研究参与肠道固有免疫和肠道抵御病原体感染的基因。如需详细了解本方案的使用和实施，请参考 Liu 等人和 Zheng 等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b0c/11111813/18551d2b23ea/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b0c/11111813/07a80ccf0677/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b0c/11111813/4b6da7551f1b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b0c/11111813/7bb3de8ce993/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b0c/11111813/c2d23fd00739/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b0c/11111813/18551d2b23ea/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b0c/11111813/07a80ccf0677/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b0c/11111813/4b6da7551f1b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b0c/11111813/7bb3de8ce993/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b0c/11111813/c2d23fd00739/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b0c/11111813/18551d2b23ea/gr4.jpg

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