Polish Academy of Sciences, Institute of Animal Reproduction and Food Research, Olsztyn, Poland.
Non-Public Health Care Clinic "ATARAX", Olsztyn, Poland.
PLoS One. 2024 May 20;19(5):e0301477. doi: 10.1371/journal.pone.0301477. eCollection 2024.
Food allergy is widely recognized as a significant health issue, having escalated into a global epidemic, subsequently giving rise to the development of numerous additional complications. Currently, the sole efficient method to curb the progression of allergy is through the implementation of an elimination diet. The increasing number of newly identified allergens makes it harder to completely remove or avoid them effectively. The immunoreactivity of proteins of bacterial origin remains an unexplored topic. Despite the substantial consumption of microbial proteins in our diets, the immunologic mechanisms they might induce require thorough validation. This stands as the primary objective of this study. The primary objective of this study was to evaluate the effects of bacterial proteins on the intestinal barrier and immune system parameters during hypersensitivity induction in both developing and mature organisms. The secondary objective was to evaluate the role of lipids in the immunoreactivity programming of these bacterial proteins. Notably, in this complex, comprehensively designed in vitro, in vivo, and ex vivo trial, the immunoreactivity of various bacterial proteins will be examined. In summary, the proposed study intends to address the knowledge gaps regarding the effects of Lactobacillus microbial proteins on inflammation, apoptosis, autophagy, and intestinal barrier integrity in a single study.
食物过敏被广泛认为是一个严重的健康问题，已经升级为全球性的流行病，随后引发了许多其他并发症。目前，抑制过敏进展的唯一有效方法是通过实施消除饮食。越来越多的新发现的过敏原使得完全去除或有效避免它们变得更加困难。细菌来源蛋白质的免疫原性仍然是一个未被探索的课题。尽管我们的饮食中大量摄入微生物蛋白，但它们可能诱导的免疫机制仍需要深入验证。这是本研究的主要目标。本研究的主要目的是评估细菌蛋白在发育和成熟生物体过敏诱导过程中对肠道屏障和免疫系统参数的影响。次要目标是评估脂质在这些细菌蛋白免疫原性编程中的作用。值得注意的是，在这个复杂的、全面设计的体外、体内和离体试验中，将检查各种细菌蛋白的免疫原性。总之，拟议的研究旨在解决关于微生物乳杆菌蛋白对炎症、细胞凋亡、自噬和肠道屏障完整性影响的单一研究中的知识空白。
