Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
Department of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
Nutrients. 2021 May 31;13(6):1887. doi: 10.3390/nu13061887.
The colonic epithelium is never exposed to a single factor, therefore studies on the effect of combinations of factors naturally and persistently present in the intestines are of special importance for understanding the phenomena occurring at this place. The aim of the study was to investigate the combined effect of 1 mM phytate and 1 mM butyrate (PA1B1) on cell lines derived from cancer (HCT116 and HT-29) and healthy (NCM460D) human colonic epithelium. Colorimetric and flow cytometry methods were used to determine the proliferation rate, cell cycle, and apoptosis. Selected markers of proliferation, inflammatory, and survival pathways were investigated at the mRNA and/or protein level. The combination of phytate and butyrate disturbed the cell cycle and triggered apoptosis and/or death in both studied cancer colonocytes to a higher extent compared to healthy colonocytes. Moreover, in healthy colonocytes, phytate activated the survival pathway without stimulation of inflammatory response. This may indicate that the response of healthy colonocytes to phytate protects colonic epithelium from the loss of integrity and tightness that would occur if inflammation developed. Based on the obtained results we postulate that studies on both cancer and/or healthy colonocytes should be carried out in the presence of butyrate as the permanent component of colonic contents. This should be of special importance when anti-proliferative/pro-apoptotic activity or inflammatory status of colonocytes is to be investigated.
结肠上皮组织不会接触到单一因素，因此，研究肠道中自然存在且持续存在的多种因素的组合对理解该部位发生的现象具有特殊意义。本研究旨在研究 1mM 植酸钠和 1mM 丁酸盐（PA1B1）对来源于癌症（HCT116 和 HT-29）和健康（NCM460D）人结肠上皮的细胞系的联合作用。采用比色法和流式细胞术测定细胞增殖率、细胞周期和细胞凋亡。在 mRNA 和/或蛋白水平研究了增殖、炎症和存活途径的选定标志物。与健康结肠细胞相比，植酸钠和丁酸盐的组合更能扰乱细胞周期，引发两种研究的癌细胞系发生凋亡和/或死亡。此外，在健康结肠细胞中，植酸钠在没有炎症反应刺激的情况下激活了存活途径。这表明健康结肠细胞对植酸钠的反应可防止结肠上皮完整性和紧密性的丧失，如果发生炎症则会出现这种丧失。基于获得的结果，我们假设在丁酸盐（结肠内容物的永久成分）存在的情况下，应同时对癌细胞系和/或健康结肠细胞系进行研究。当要研究结肠细胞的抗增殖/促凋亡活性或炎症状态时，这一点尤为重要。
