College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.
The Affiliated Suqian First People's Hospital of Nanjing Medical University, Suzhi Road 120, Suqian 223800, PR China.
J Tissue Viability. 2024 Aug;33(3):487-503. doi: 10.1016/j.jtv.2024.05.006. Epub 2024 May 16.
Severe bacterial infections can give rise to protracted wound healing processes, thereby posing a significant risk to a patient's well-being. Consequently, the development of a versatile hydrogel dressing possessing robust bioactivity becomes imperative, as it holds the potential to expedite wound healing and yield enhanced clinical therapeutic outcomes. In this context, the present study involves the formulation of an injectable multifunctional hydrogel utilizing laponite (LAP) and lactoferrin (LF) as foundational components and loaded with eugenol (EG). This hydrogel is fabricated employing a straightforward one-pot mixing approach that leverages the principle of electrostatic interaction. The resulting LAP/LF/EG composite hydrogel can be conveniently injected to address irregular wound geometries effectively. Once administered, the hydrogel continually releases lactoferrin and eugenol, mitigating unwarranted oxidative stress and eradicating bacterial infections. This orchestrated action culminates in the acceleration of wound healing specifically in the context of MRSA-infected wounds. Importantly, the LAP/LF/EG hydrogel exhibits commendable qualities including exceptional injectability, potent antioxidant attributes, and proficient hemostatic functionality. Furthermore, the hydrogel composition notably encourages cellular migration while maintaining favorable cytocompatibility. Additionally, the hydrogel manifests noteworthy bactericidal efficacy against the formidable multidrug-resistant MRSA bacterium. Most significantly, this hydrogel formulation distinctly expedites the healing of MRSA-infected wounds by promptly inducing hemostasis, curbing bacterial proliferation, and fostering angiogenesis, collagen deposition, and re-epithelialization processes. As such, the innovative hydrogel material introduced in this investigation emerges as a promising dressing for the facilitation of bacterial-infected wound healing and consequent tissue regeneration.
严重的细菌感染可能导致伤口愈合过程延长，从而对患者的健康构成重大威胁。因此，开发一种多功能水凝胶敷料至关重要，这种敷料具有强大的生物活性，能够加速伤口愈合，并带来更好的临床治疗效果。在这种情况下，本研究利用纳米片（LAP）和乳铁蛋白（LF）作为基础成分，制备了一种可注射的多功能水凝胶，并负载了丁香酚（EG）。这种水凝胶是通过简单的一锅混合方法制备的，利用静电相互作用的原理。所得的 LAP/LF/EG 复合水凝胶可以方便地注射到不规则的伤口中，以有效地解决问题。一旦给药，水凝胶会持续释放乳铁蛋白和丁香酚，减轻不必要的氧化应激并消除细菌感染。这种协同作用最终加速了伤口愈合，特别是在耐甲氧西林金黄色葡萄球菌（MRSA）感染的伤口中。重要的是，LAP/LF/EG 水凝胶具有出色的可注射性、强大的抗氧化特性和高效的止血功能等优点。此外，水凝胶的组成明显促进了细胞迁移，同时保持了良好的细胞相容性。此外，水凝胶对强大的多药耐药性 MRSA 细菌表现出显著的杀菌效果。最重要的是，这种水凝胶配方通过迅速止血、抑制细菌增殖、促进血管生成、胶原沉积和上皮再形成等过程，明显加速了 MRSA 感染伤口的愈合。因此，本研究中引入的创新水凝胶材料有望成为促进细菌感染性伤口愈合和随后的组织再生的理想敷料。
